FastQCFastQC Report
Thu 26 May 2016
SRR1512498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130284
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62010.29108794883686867No Hit
GTCCTAAAGTGTGTATTTCTCATTT60810.28545489709353306No Hit
CTGTAGGACGTGGAATATGGCAAGA59290.2783196982186413No Hit
CTTTAGGACGTGAAATATGGCGAGG52730.24752568202173983No Hit
GTATCAACGCAGAGTACTTTTTTTT51490.2417048618869597No Hit
GTACATGGGAAGCAGTGGTATCAAC44830.21044142471144692No Hit
CCCATGTACTCTGCGTTGATACCAC32390.1520454549722009No Hit
GGTATCAACGCAGAGTACTTTTTTT31440.1475859556753935No Hit
GTCCTACAGTGTGCATTTCTCATTT31100.14598992434811509No Hit
TATCAACGCAGAGTACTTTTTTTTT30720.14420612462939214No Hit
CTGAAGGACCTGGAATATGGCGAGA26410.123974080451245No Hit
GTCCTTCAGTGTGCATTTCTCATTT24850.11665111318490869No Hit
ATTTAGAAATGTCCACTGTAGGACG23020.10806070927632185No Hit
CTGTAGGACCTGGAATATGGCGAGA22370.10500947291534837No Hit
TTTCTAAATTTTCCACCTTTTTCAG21530.10106633669501343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9600.012.5380954
TGGACCG550.003123778812.062425
CCGCTCA550.003130958912.0587229
GGCGAGG17400.011.81476719
GTGTAGG3500.011.6049361
AGGACGT38850.011.3926535
GGACGTG38700.011.3388516
GACGTGG18500.011.1169867
GACGTGA21900.010.9490257
GGCTTAT1551.8353603E-910.865361
GGACCGA700.001521434610.831566
TCGAACT1053.5851226E-610.82696219
TTAGGAC24350.010.8204383
GTCCTAC30650.010.7631811
TGTAGGA35500.010.7593492
GCGCCAC803.8323828E-410.66911113
CCGTGCA803.865942E-410.6590499
TAGGACG43600.010.6514484
ATTGCGA909.632327E-510.54311817
CACCTTT8100.010.5426214
Produced by FastQC (version 0.11.5)