FastQCFastQC Report
Thu 26 May 2016
SRR1512498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130284
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA70990.33324195271616364No Hit
GTATCAACGCAGAGTACTTTTTTTT66870.313901808397378No Hit
GTCCTACAGTGGACATTTCTAAATT66370.3115547035043215No Hit
GTCCTAAAGTGTGTATTTCTCATTT65260.30634413064173605No Hit
CTTTAGGACGTGAAATATGGCGAGG63780.2993967001582888No Hit
GTACATGGGAAGCAGTGGTATCAAC53050.249027829153296No Hit
TATCAACGCAGAGTACTTTTTTTTT45370.21297629799594797No Hit
GGTATCAACGCAGAGTACTTTTTTT44830.21044142471144692No Hit
CCCATGTACTCTGCGTTGATACCAC36910.17326328320543177No Hit
GTCCTACAGTGTGCATTTCTCATTT33880.15903982755350932No Hit
CTGAAGGACCTGGAATATGGCGAGA33650.1579601593027033No Hit
ATTTAGAAATGTCCACTGTAGGACG28620.13434828407855479No Hit
CTGTAGGACCTGGAATATGGCGAGA27770.13035820576035873No Hit
TTTCTAAATTTTCCACCTTTTTCAG27540.12927853750955273No Hit
GTCCTTCAGTGTGCATTTCTCATTT25850.1213453229710217No Hit
GAATATGGCAAGAAAACTGAAAATC24570.11533673444479703No Hit
GAGTACATGGGAAGCAGTGGTATCA23380.10975062479932254No Hit
GGAATATGGCGAGAAAACTGAAAAT22400.10515029920893178No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22190.10416451515384802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC250.006036672718.9978878
CCACCTT9200.011.56311713
ACCTTTT9300.011.53955915
GGCGAGG18500.011.3957919
TAGGACC9900.011.3270264
TAGAAAT10100.011.2909114
AAATGTC10350.011.1970857
TAGGACT4650.011.0359544
CACCTTT9650.010.92419414
TCCAACG2000.010.92096418
TTTAGAA11550.010.8607812
GTTCTAA1951.8189894E-1210.7354081
CCAACGA1158.8625166E-710.73516419
AATGTCC10550.010.7144488
GAAATGT10950.010.5835476
AGGACGT38900.010.4315145
TTAGGAC25800.010.38723
GGACGTG38700.010.3582646
TAATACT2850.010.3367964
GACGTGA22650.010.3169767
Produced by FastQC (version 0.11.5)