FastQCFastQC Report
Thu 26 May 2016
SRR1512497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60450.28612025665152685No Hit
GTCCTACAGTGGACATTTCTAAATT57380.27158941814168086No Hit
GTCCTAAAGTGTGTATTTCTCATTT51860.24546230785687642No Hit
CTGTAGGACGTGGAATATGGCAAGA50330.23822055446271867No Hit
CTTTAGGACGTGAAATATGGCGAGG44450.21038950220281832No Hit
GTACATGGGAAGCAGTGGTATCAAC43930.20792825268323528No Hit
GGTATCAACGCAGAGTACTTTTTTT36390.17224013464928142No Hit
TATCAACGCAGAGTACTTTTTTTTT33860.16026520910207936No Hit
CCCATGTACTCTGCGTTGATACCAC31870.15084619651752126No Hit
GTCCTACAGTGTGCATTTCTCATTT31340.1483376152764078No Hit
CTGAAGGACCTGGAATATGGCGAGA21320.10091123030290408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8900.012.7810244
TACGGTG550.003119706612.0645125
ACACCGT802.9323313E-511.8490756
TACACCG658.173964E-411.666785
AGTCGTC2200.011.1737778
CACCGTT600.00597319511.0591367
GTGCGCC1201.315475E-711.05887311
GGCGAGG13200.011.04914519
TCCAACG951.4095918E-510.96502518
GTCCTAA17900.010.7187371
AATGTCC8150.010.672838
CCAACGA803.8810773E-410.65453219
AGGACCT17650.010.6339575
GGACGTG31950.010.6215186
AAGTCGT2500.010.6167717
GTCCTAC28600.010.5124151
AGGACGT32850.010.50365455
TGTAGGA33600.010.4666832
ATAGGAC3750.010.3639923
TTAGGAC19100.010.27333453
Produced by FastQC (version 0.11.5)