FastQCFastQC Report
Thu 26 May 2016
SRR1512497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112748
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75280.35631319968117353No Hit
CTGTAGGACGTGGAATATGGCAAGA63370.2999411193384161No Hit
GTCCTACAGTGGACATTTCTAAATT60870.2881081889558054No Hit
GTCCTAAAGTGTGTATTTCTCATTT56200.26600427500108864No Hit
CTTTAGGACGTGAAATATGGCGAGG54330.25715324307489584No Hit
GTACATGGGAAGCAGTGGTATCAAC51970.24598295679371132No Hit
GGTATCAACGCAGAGTACTTTTTTT48670.23036348868866519No Hit
TATCAACGCAGAGTACTTTTTTTTT48280.22851755154897793No Hit
CCCATGTACTCTGCGTTGATACCAC38970.18445171880413566No Hit
GTCCTACAGTGTGCATTTCTCATTT34890.165140376419715No Hit
CTGAAGGACCTGGAATATGGCGAGA27930.13219749823452678No Hit
CTGTAGGACCTGGAATATGGCGAGA24650.11667269357254154No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23140.10952560362144467No Hit
GAGTACATGGGAAGCAGTGGTATCA22240.10526574868370484No Hit
ATTTAGAAATGTCCACTGTAGGACG22230.10521841696217438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG400.00529076914.24559716
TAGGACC10350.012.9463044
AATCCCG1352.7284841E-1112.66065519
CCGTGCT550.003070734212.0900359
GGCGAGG14900.011.98088819
CGGCGAG658.0527406E-411.68758918
CCTACAC2850.011.6702443
TGTAGGA33750.011.544282
GACGTGG17100.011.3883357
GGACGTG33850.011.3374386
AGGACGT34350.011.2599055
CTGTAGG33050.011.2262231
ATACGGC855.337648E-511.17381115
CATGCGC600.005880004711.08253310
ATTTAGA8050.010.9907571
GTAGGAC33050.010.8974413
TGGACCG700.001487680810.860735
CTAACGT700.001487680810.860734
AGCCGTG700.001492390910.8566157
GGACCTG19150.010.813846
Produced by FastQC (version 0.11.5)