FastQCFastQC Report
Thu 26 May 2016
SRR1512496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103672
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71380.3393114515951156No Hit
GTCCTAAAGTGTGTATTTCTCATTT67390.32034461646112133No Hit
CTGTAGGACGTGGAATATGGCAAGA67290.3198692571845801No Hit
GTACATGGGAAGCAGTGGTATCAAC66010.3137846584448526No Hit
CTTTAGGACGTGAAATATGGCGAGG55430.2634916469867926No Hit
GTATCAACGCAGAGTACTTTTTTTT48820.23207039880741867No Hit
CCCATGTACTCTGCGTTGATACCAC48180.2290280994375549No Hit
GTCCTACAGTGTGCATTTCTCATTT33600.1597207169178465No Hit
CTGAAGGACCTGGAATATGGCGAGA31500.1497381721104811No Hit
GGTATCAACGCAGAGTACTTTTTTT29910.14217995961347585No Hit
TATCAACGCAGAGTACTTTTTTTTT28810.13695100757152256No Hit
GTCCTTCAGTGTGCATTTCTCATTT28510.13552492974189892No Hit
GAGTACATGGGAAGCAGTGGTATCA28210.1340988519122753No Hit
ATTTAGAAATGTCCACTGTAGGACG27100.12882236394266788No Hit
CTGTAGGACCTGGAATATGGCGAGA23310.11080624736175602No Hit
TTTCTAAATTTTCCACCTTTTTCAG22720.10800162763016288No Hit
GAATATGGCAAGAAAACTGAAAATC22450.10671815758350162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10450.013.3309844
GGCGAGG16900.011.655663519
TCGAACT1157.392555E-811.52897219
GTATAGA2700.011.01258851
CCTACAC3800.010.9731243
AGGACCT24500.010.9466575
GGACGTG39550.010.758736
TCTATAC1855.456968E-1210.7574363
AGGACGT40050.010.7190645
ACCTTTT9850.010.69296715
CTAGGAC2750.010.6829253
AAGGCGT803.8580716E-410.66138656
TGTAGGA38000.010.6491722
CCACCTT9900.010.6336213
GACGTGG20150.010.6290537
GTCCTAA21450.010.58132651
GGACCTG24100.010.5778266
GTCTTAT2250.010.5720851
CACCTTT10200.010.51210614
GATCAAC1801.8189894E-1010.4619591
Produced by FastQC (version 0.11.5)