FastQCFastQC Report
Thu 26 May 2016
SRR1512496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103672
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA80440.3823790020497492No Hit
GTACATGGGAAGCAGTGGTATCAAC78060.37106545126806845No Hit
GTCCTACAGTGGACATTTCTAAATT76440.3633646309881008No Hit
GTCCTAAAGTGTGTATTTCTCATTT71900.3417833198331299No Hit
CTTTAGGACGTGAAATATGGCGAGG67500.32086751166531663No Hit
GTATCAACGCAGAGTACTTTTTTTT64350.3058936944542685No Hit
CCCATGTACTCTGCGTTGATACCAC56810.2700516050030613No Hit
TATCAACGCAGAGTACTTTTTTTTT42590.20245551587890126No Hit
GGTATCAACGCAGAGTACTTTTTTT40950.19465962374362544No Hit
CTGAAGGACCTGGAATATGGCGAGA39570.18809966572735673No Hit
GTCCTACAGTGTGCATTTCTCATTT36640.17417163892469928No Hit
GAGTACATGGGAAGCAGTGGTATCA33470.15910274985834294No Hit
ATTTAGAAATGTCCACTGTAGGACG31590.15016599545936818No Hit
GTCCTTCAGTGTGCATTTCTCATTT29190.13875737282237915No Hit
TTTCTAAATTTTCCACCTTTTTCAG28820.13699854349917667No Hit
CTGTAGGACCTGGAATATGGCGAGA28060.1333858129974635No Hit
GAATATGGCAAGAAAACTGAAAATC26400.1254948490068794No Hit
GGAATATGGCGAGAAAACTGAAAAT22700.10790655577485463No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22370.10633787016226864No Hit
GTATCAACGCAGAGTACATGGGAAG21560.10248746002228484No Hit
CATGTACTCTGCGTTGATACCACTG21340.10144166961389418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC551.9605868E-413.81825211
TAGGACC11100.012.4132194
GCGCCAC1001.444605E-712.34918113
TGTCCAC10800.011.78709610
ATGTCCA11750.011.4809089
GGCGAGG16400.011.46466319
TGCGCCA1001.9308918E-611.39978712
TCCAACG2500.011.39545118
GAAATGT11450.011.200936
TAGGACT3650.011.1947654
GTCGAAA600.005832028611.0947331
AAATGTC11250.010.97757157
AATGTCC11550.010.9391898
ACCTTTT9400.010.912353515
TAGAAAT12550.010.9033054
GATATAC6550.010.8890511
TAGTACG700.001488467310.8600384
GTACCGT700.001491719410.8571986
CCAACGA1502.1482265E-910.76211519
AGGACGA1158.7873195E-710.74224955
Produced by FastQC (version 0.11.5)