##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103672 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.525361843481303 33.0 31.0 34.0 30.0 34.0 2 31.806489319627776 33.0 31.0 34.0 30.0 34.0 3 31.977378602747958 34.0 31.0 34.0 30.0 34.0 4 35.45051937754555 37.0 35.0 37.0 33.0 37.0 5 34.92291098612331 37.0 35.0 37.0 32.0 37.0 6 35.16155988195879 37.0 35.0 37.0 33.0 37.0 7 35.11935368251324 37.0 35.0 37.0 32.0 37.0 8 35.09195682596907 37.0 35.0 37.0 32.0 37.0 9 36.68967405565126 39.0 37.0 39.0 33.0 39.0 10 36.62244779604425 39.0 37.0 39.0 32.0 39.0 11 36.66037338520454 39.0 37.0 39.0 33.0 39.0 12 36.590414760475966 39.0 37.0 39.0 32.0 39.0 13 36.55863033780932 39.0 37.0 39.0 32.0 39.0 14 37.83282089603322 40.0 38.0 41.0 32.0 41.0 15 37.83344361668549 40.0 38.0 41.0 32.0 41.0 16 37.80948408306998 40.0 38.0 41.0 32.0 41.0 17 37.793491570929305 40.0 38.0 41.0 32.0 41.0 18 37.74223215406204 40.0 38.0 41.0 32.0 41.0 19 37.75902279442803 40.0 38.0 41.0 32.0 41.0 20 37.75287164538958 40.0 38.0 41.0 32.0 41.0 21 37.69714575275994 40.0 38.0 41.0 32.0 41.0 22 37.641531094201 40.0 38.0 41.0 32.0 41.0 23 37.53246038355789 40.0 38.0 41.0 32.0 41.0 24 37.51957006605593 40.0 38.0 41.0 32.0 41.0 25 37.456957168227746 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17173114717973448 1101 2 0.2538147604176828 1101 3 0.1889020502635823 1101 4 0.2556285248131758 1101 5 -0.003418290957682757 1101 6 0.2501062914877892 1101 7 0.35211727885040744 1101 8 0.44854758122474436 1101 9 0.49131530460454087 1101 10 0.37729646073304934 1101 11 0.3813743166004073 1101 12 0.3798461941512983 1101 13 0.47962133006485175 1101 14 0.5943394987628139 1101 15 0.6232190036393703 1101 16 0.5278012065868367 1101 17 0.6197002503465683 1101 18 0.5015044446699264 1101 19 0.5941503909319152 1101 20 0.532113470241903 1101 21 0.5629435662601807 1101 22 0.5737294900614245 1101 23 0.6935156742102038 1101 24 0.546045484923475 1101 25 0.5931654706740375 1102 1 0.12416785611314651 1102 2 0.2928122051276816 1102 3 0.3123572247832307 1102 4 0.3159935203247741 1102 5 0.022864691912921842 1102 6 0.22070553339546706 1102 7 0.25500018779437994 1102 8 0.3500254250467023 1102 9 0.2321221150378321 1102 10 0.3527468506845395 1102 11 0.30595997162018307 1102 12 0.3531430692589197 1102 13 0.4164921214700712 1102 14 0.5252318758253551 1102 15 0.6722516438531656 1102 16 0.5534803787922584 1102 17 0.5421094319420519 1102 18 0.617169772851355 1102 19 0.6320986285164878 1102 20 0.6565512720037461 1102 21 0.5595296253509758 1102 22 0.6123182720398574 1102 23 0.5664491149896875 1102 24 0.5561794023230959 1102 25 0.5807127366514848 1103 1 -0.183627859675628 1103 2 0.0024159816097508724 1103 3 -0.044917886390809514 1103 4 0.09423154839028314 1103 5 -0.1399258946044739 1103 6 0.02380902637848692 1103 7 0.06838671976763777 1103 8 0.21222218712319574 1103 9 0.31982578475219015 1103 10 0.22713198039804183 1103 11 0.31775520465400575 1103 12 0.3436964868712309 1103 13 0.32282033389659404 1103 14 0.43855226798010705 1103 15 0.5260527715161558 1103 16 0.3030781327867871 1103 17 0.3527619457630067 1103 18 0.3575204991048011 1103 19 0.40462604786265643 1103 20 0.34638957896778066 1103 21 0.420425099941518 1103 22 0.40761583251417477 1103 23 0.38313785813597434 1103 24 0.4388263333936919 1103 25 0.4314082204430463 1104 1 -0.23489683759412472 1104 2 -0.11092057252496801 1104 3 -0.04602080287994781 1104 4 0.024349666230016 1104 5 -0.4412556339513145 1104 6 -0.10400449516374266 1104 7 -0.1416529103512545 1104 8 -0.07354193017815192 1104 9 0.030428460869714513 1104 10 -0.029643735947999517 1104 11 -0.07768492498622237 1104 12 0.012751419362160732 1104 13 -0.07741058924332123 1104 14 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-0.6634461360923609 2314 13 -0.9553733557509787 2314 14 -2.4265497344285976 2314 15 -1.6538410518620097 2314 16 -1.3518121630235456 2314 17 -1.142669061440479 2314 18 -0.9380610659615698 2314 19 -0.9535989633268471 2314 20 -1.0089017999462584 2314 21 -0.889252789190877 2314 22 -0.819557724366426 2314 23 -0.8086089554151741 2314 24 -0.6816646968028337 2314 25 -0.7779566887431386 2315 1 -0.8581944587249204 2315 2 -0.6792671302601576 2315 3 -0.5013419522129645 2315 4 -0.42717127412266365 2315 5 -0.17512555900812998 2315 6 -0.4334625015217668 2315 7 -0.5891327102742991 2315 8 -0.93845121654509 2315 9 -0.689181507115137 2315 10 -0.7799021920447302 2315 11 -0.7690346032336919 2315 12 -0.7061094456682824 2315 13 -0.6713282471126973 2315 14 -0.8317359521445269 2315 15 -0.8901110851580469 2315 16 -0.9790413492007417 2315 17 -0.9582058095078381 2315 18 -1.0781068608746196 2315 19 -1.016028917321428 2315 20 -0.8277013603885308 2315 21 -0.7157023507648077 2315 22 -0.7328731168480189 2315 23 -0.7926142898720414 2315 24 -0.984268346689376 2315 25 -0.8880533005938176 2316 1 -0.46436063060316 2316 2 -0.5603055968003048 2316 3 -0.6345785153889736 2316 4 -0.5192794915382564 2316 5 -0.272481830698041 2316 6 -0.7088589976923174 2316 7 -1.185252491795275 2316 8 -0.8685303044076207 2316 9 -0.9744653616952519 2316 10 -1.0807958710683394 2316 11 -1.0464626822096292 2316 12 -1.063350894949906 2316 13 -0.973125254440113 2316 14 -1.1457900952085893 2316 15 -1.206411135095422 2316 16 -1.2667920107266752 2316 17 -1.3639325432345402 2316 18 -1.3548291600927627 2316 19 -1.4375862413599592 2316 20 -1.5162393709699913 2316 21 -1.592142177034816 2316 22 -1.5026263945501412 2316 23 -1.4837119766886175 2316 24 -1.5733042950223748 2316 25 -1.4316712009077648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18246.0 3 0.0 4 417.0 5 2978.0 6 3763.0 7 2977.0 8 2397.0 9 2211.0 10 2046.0 11 1880.0 12 1765.0 13 1743.0 14 1733.0 15 1763.0 16 1846.0 17 1895.0 18 2271.0 19 2482.0 20 3126.0 21 4114.0 22 5213.0 23 6624.0 24 8171.0 25 9747.0 26 11334.0 27 13121.0 28 15163.0 29 18327.0 30 23910.0 31 31347.0 32 40920.0 33 54367.0 34 73603.0 35 101933.0 36 153112.0 37 285796.0 38 1191331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87469984345453 19.746147881924067 13.020832589635253 25.358319684986153 2 13.830445176842836 21.16219360132269 42.54001847317278 22.467342748661693 3 18.721091746773922 25.868519203399064 30.369293576176133 25.041095473650888 4 11.968980735844715 17.825951370890607 39.15186185940646 31.05320603385821 5 11.937259967015972 39.05568479900477 36.78950712276183 12.217548111217424 6 30.55922339883782 38.258191547482234 18.7301395597859 12.452445493894052 7 26.8674648958107 31.943730598377073 23.30876329318732 17.88004121262491 8 23.982732554269795 39.071304281679964 21.094761764398253 15.851201399651988 9 26.614212985590374 14.653195240277734 20.81166120544892 37.92093056868298 10 14.643387978246006 29.53635349574972 35.69258361489458 20.127674911109693 11 33.159397052593135 21.995854629591328 26.4638012352424 18.380947082573133 12 23.748166357988733 24.812189885666328 33.200510685645405 18.239133070699527 13 29.41236606535754 22.3991692176895 26.01848338482928 22.16998133212368 14 19.66089865706816 23.795238432563163 28.238414021602342 28.305448888766332 15 22.170656817838196 33.46801301977595 23.242325492496892 21.119004669888962 16 20.11185136690586 28.398389789129318 30.841424086696932 20.648334757267893 17 20.735974532516114 29.838099295042092 29.138941555243253 20.286984617198538 18 20.453674607438117 27.355062301223203 32.746007825090686 19.445255266247997 19 23.24176393577909 26.858492099786368 28.452700923268242 21.447043041166296 20 23.21400983521616 28.176824732074735 29.230509610608152 19.378655822100953 21 21.493044034640548 27.146756465629934 28.2932809548302 23.066918544899323 22 22.41055974702547 29.732778541279732 27.794825183933717 20.061836527761074 23 20.439934361143013 29.458384356199186 29.24798633981609 20.853694942841713 24 22.618114989912666 27.712979671310705 29.914319451918303 19.754585886858322 25 21.589518122860582 28.189035442362844 29.245665549095413 20.97578088568116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 262.0 1 262.0 2 515.0 3 768.0 4 768.0 5 768.0 6 1897.5 7 3027.0 8 3027.0 9 3027.0 10 4006.0 11 4985.0 12 4985.0 13 4985.0 14 7556.0 15 10127.0 16 10127.0 17 10127.0 18 18598.0 19 27069.0 20 27069.0 21 27069.0 22 44721.0 23 62373.0 24 62373.0 25 62373.0 26 91799.5 27 121226.0 28 121226.0 29 121226.0 30 158995.0 31 196764.0 32 196764.0 33 196764.0 34 215402.0 35 234040.0 36 234040.0 37 234040.0 38 242552.5 39 251065.0 40 251065.0 41 251065.0 42 258506.5 43 265948.0 44 265948.0 45 265948.0 46 277398.0 47 288848.0 48 288848.0 49 288848.0 50 265118.0 51 241388.0 52 241388.0 53 241388.0 54 202124.5 55 162861.0 56 162861.0 57 162861.0 58 135667.5 59 108474.0 60 108474.0 61 108474.0 62 87362.0 63 66250.0 64 66250.0 65 66250.0 66 50330.0 67 34410.0 68 34410.0 69 34410.0 70 24635.5 71 14861.0 72 14861.0 73 14861.0 74 10319.0 75 5777.0 76 5777.0 77 5777.0 78 3963.0 79 2149.0 80 2149.0 81 2149.0 82 1431.0 83 713.0 84 713.0 85 713.0 86 473.5 87 234.0 88 234.0 89 234.0 90 138.0 91 42.0 92 42.0 93 42.0 94 25.5 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12763396575131483 2 0.0038504101399838 3 0.0016162215402401134 4 0.003612730501713195 5 0.03974003551884514 6 0.02657258355865363 7 0.020583056674234386 8 0.013880490875003327 9 0.01692279024486707 10 0.02462361052483467 11 0.028664164375434953 12 0.029329667362592647 13 0.028236341026547866 14 0.013737883092040964 15 0.019489730338189605 16 0.01639989504067174 17 0.01350020345377036 18 0.00865153883305002 19 0.002757083803939017 20 3.327514935788469E-4 21 4.753592765412098E-4 22 0.0028996915869013797 23 0.0019014371061648393 24 0.0020915808167813236 25 1.4260778296236297E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2103672.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.98608146833494 #Duplication Level Percentage of deduplicated Percentage of total 1 85.68737695977792 58.255489907953894 2 10.2029728642946 13.87320288742287 3 2.1349340897679276 4.354374088694636 4 0.7190674964563626 1.9554632558125569 5 0.3491888423179066 1.1869990540829383 6 0.19166384617463447 0.7818284316337871 7 0.1333047743547478 0.6344008474579925 8 0.08170407975359134 0.44437921779383976 9 0.06601807210097049 0.40394790254150914 >10 0.3734338738167498 4.6274976014706946 >50 0.028303349019479094 1.3157149773790895 >100 0.024061265134989355 3.438596038577525 >500 0.0040551600679557855 1.8788100937974184 >1k 0.0034259110918936305 4.4940095508774265 >5k 4.894158702705187E-4 2.3552861445038977 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 8044 0.3823790020497492 No Hit GTACATGGGAAGCAGTGGTATCAAC 7806 0.37106545126806845 No Hit GTCCTACAGTGGACATTTCTAAATT 7644 0.3633646309881008 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7190 0.3417833198331299 No Hit CTTTAGGACGTGAAATATGGCGAGG 6750 0.32086751166531663 No Hit GTATCAACGCAGAGTACTTTTTTTT 6435 0.3058936944542685 No Hit CCCATGTACTCTGCGTTGATACCAC 5681 0.2700516050030613 No Hit TATCAACGCAGAGTACTTTTTTTTT 4259 0.20245551587890126 No Hit GGTATCAACGCAGAGTACTTTTTTT 4095 0.19465962374362544 No Hit CTGAAGGACCTGGAATATGGCGAGA 3957 0.18809966572735673 No Hit GTCCTACAGTGTGCATTTCTCATTT 3664 0.17417163892469928 No Hit GAGTACATGGGAAGCAGTGGTATCA 3347 0.15910274985834294 No Hit ATTTAGAAATGTCCACTGTAGGACG 3159 0.15016599545936818 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2919 0.13875737282237915 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2882 0.13699854349917667 No Hit CTGTAGGACCTGGAATATGGCGAGA 2806 0.1333858129974635 No Hit GAATATGGCAAGAAAACTGAAAATC 2640 0.1254948490068794 No Hit GGAATATGGCGAGAAAACTGAAAAT 2270 0.10790655577485463 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2237 0.10633787016226864 No Hit GTATCAACGCAGAGTACATGGGAAG 2156 0.10248746002228484 No Hit CATGTACTCTGCGTTGATACCACTG 2134 0.10144166961389418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.7535927654120984E-5 2 0.0 0.0 0.0 0.0 4.7535927654120984E-5 3 0.0 0.0 0.0 0.0 4.7535927654120984E-5 4 0.0 0.0 0.0 0.0 4.7535927654120984E-5 5 0.0 0.0 0.0 0.0 4.7535927654120984E-5 6 0.0 0.0 0.0 0.0 4.7535927654120984E-5 7 0.0 0.0 0.0 0.0 4.7535927654120984E-5 8 0.0 0.0 0.0 0.0 4.7535927654120984E-5 9 0.0 0.0 0.0 0.0 4.7535927654120984E-5 10 0.0 0.0 0.0 0.0 4.7535927654120984E-5 11 0.0 0.0 0.0 0.0 4.7535927654120984E-5 12 0.0 0.0 0.0 4.7535927654120984E-5 4.7535927654120984E-5 13 0.0 0.0 0.0 9.507185530824197E-5 4.7535927654120984E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGCC 55 1.9605868E-4 13.818252 11 TAGGACC 1110 0.0 12.413219 4 GCGCCAC 100 1.444605E-7 12.349181 13 TGTCCAC 1080 0.0 11.787096 10 ATGTCCA 1175 0.0 11.480908 9 GGCGAGG 1640 0.0 11.464663 19 TGCGCCA 100 1.9308918E-6 11.399787 12 TCCAACG 250 0.0 11.395451 18 GAAATGT 1145 0.0 11.20093 6 TAGGACT 365 0.0 11.194765 4 GTCGAAA 60 0.0058320286 11.094733 1 AAATGTC 1125 0.0 10.9775715 7 AATGTCC 1155 0.0 10.939189 8 ACCTTTT 940 0.0 10.9123535 15 TAGAAAT 1255 0.0 10.903305 4 GATATAC 655 0.0 10.889051 1 TAGTACG 70 0.0014884673 10.860038 4 GTACCGT 70 0.0014917194 10.857198 6 CCAACGA 150 2.1482265E-9 10.762115 19 AGGACGA 115 8.7873195E-7 10.7422495 5 >>END_MODULE