FastQCFastQC Report
Thu 26 May 2016
SRR1512495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3040348
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72520.2385253267060218No Hit
CTGTAGGACGTGGAATATGGCAAGA66170.21763955968198379No Hit
GTCCTAAAGTGTGTATTTCTCATTT64230.21125871117385248No Hit
CTTTAGGACGTGAAATATGGCGAGG52940.1741248041342636No Hit
GTATCAACGCAGAGTACTTTTTTTT51480.1693227222673194No Hit
GTCCTACAGTGTGCATTTCTCATTT41960.13801051721710805No Hit
CTGTAGGACCTGGAATATGGCGAGA30970.10186333932826111No Hit
TATCAACGCAGAGTACTTTTTTTTT30770.10120551989443315No Hit
GGTATCAACGCAGAGTACTTTTTTT30570.10054770046060517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13300.013.3266394
ACCGCCC550.003181832112.0330648
GGCGAGG16400.011.83555619
TGGCGAA1657.2759576E-1211.47614518
GTCCTAA20000.011.2967461
GTGTAGG6450.011.2153211
AGTCGAG600.005977236711.05838710
AGGACCT25200.010.8323485
TAAGACG1053.5654703E-610.8323484
GTCCTAC39500.010.8125641
CACCTTT10450.010.80383514
ACCTTTT10950.010.6570815
TGTAGGA47800.010.6287432
ATTTAGA12050.010.6084321
CCACCTT10400.010.57842413
GGACGTG41400.010.5772386
AATGTCC11450.010.5692798
GGACCTG24350.010.548756
TGTCCAC12150.010.53179810
ACCGTGC909.995213E-510.5050558
Produced by FastQC (version 0.11.5)