FastQCFastQC Report
Thu 26 May 2016
SRR1512495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3040348
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA81300.2674035998510697No Hit
GTCCTACAGTGGACATTTCTAAATT77980.2564837972495254No Hit
GTATCAACGCAGAGTACTTTTTTTT68840.22642144912358714No Hit
GTCCTAAAGTGTGTATTTCTCATTT68630.2257307387180678No Hit
CTTTAGGACGTGAAATATGGCGAGG64530.21224544032459441No Hit
TATCAACGCAGAGTACTTTTTTTTT46370.1525154357330148No Hit
GTCCTACAGTGTGCATTTCTCATTT46030.15139714269550722No Hit
GGTATCAACGCAGAGTACTTTTTTT44510.14639771499841467No Hit
CTGTAGGACCTGGAATATGGCGAGA37950.12482123756885725No Hit
CTGAAGGACCTGGAATATGGCGAGA35490.11673005853277323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG1403.6379788E-1212.8961855
TAGGACC15200.012.8783244
TCCAACG2150.012.36755218
GGCGAGG17900.011.98922319
GGACGTA658.0309674E-411.6916696
GGCGAAA1451.2278178E-911.13311119
CGGTACT600.005866566711.0861944
AGGACGT42400.011.0040195
GATATAC7350.011.0029931
GGACGTG41700.010.9346556
GACGTGG20450.010.9162757
TGTAGGA47250.010.8798782
TAGAAAT11600.010.7312184
TAGAACG803.7639213E-410.6902594
AGGACCT27750.010.6838385
GTAGGAC45800.010.6434023
AATGTCC11100.010.44078
GTCCTAC38950.010.3570871
CTGTAGG46450.010.3439141
CGGTTTC1752.3646862E-1010.31253513
Produced by FastQC (version 0.11.5)