FastQCFastQC Report
Thu 26 May 2016
SRR1512494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52580.22830762737659424No Hit
CTGTAGGACGTGGAATATGGCAAGA47890.2079431775402263No Hit
GTCCTACAGTGGACATTTCTAAATT47130.20464318140469545No Hit
GTCCTAAAGTGTGTATTTCTCATTT44770.19439582498383653No Hit
CTTTAGGACGTGAAATATGGCGAGG37750.16391428173195957No Hit
GGTATCAACGCAGAGTACTTTTTTT31870.13838273268337883No Hit
TATCAACGCAGAGTACTTTTTTTTT31040.1347787895353649No Hit
GTCCTACAGTGTGCATTTCTCATTT23670.10277751122107238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAA604.1753644E-412.6369082
TAGGACC8950.012.4948954
GACAGCG1501.8371793E-1011.372727
GTGTAGG3850.011.3075221
ATTATAC2650.011.0863713
AGGACCG1159.100477E-710.7131985
CGTGCGC803.8464754E-410.6649510
GGTATCA15600.010.4648941
TGTAGGA29750.010.4493412
GGCGAGG12650.010.40852919
GGACCTG17950.010.348216
AGGACCT18600.010.3432435
GTATTAG2400.010.30633451
AATCCCG1856.366463E-1110.24055319
GTAGGAC28800.010.2010023
GACGTGG15400.010.0311327
AGGACGT30350.09.9298355
GTCCTAA14350.09.9285561
GGACGTG30150.09.9014056
TGGACAG4050.09.8280615
Produced by FastQC (version 0.11.5)