FastQCFastQC Report
Thu 26 May 2016
SRR1512494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63650.27637467635070795No Hit
CTGTAGGACGTGGAATATGGCAAGA57670.25040891728429426No Hit
GTCCTACAGTGGACATTTCTAAATT50880.22092605707343316No Hit
GTCCTAAAGTGTGTATTTCTCATTT49750.2160194838719202No Hit
CTTTAGGACGTGAAATATGGCGAGG46380.2013866062709479No Hit
TATCAACGCAGAGTACTTTTTTTTT44460.1930497739285542No Hit
GGTATCAACGCAGAGTACTTTTTTT42900.18627609765035932No Hit
GTCCTACAGTGTGCATTTCTCATTT26300.11419723469008043No Hit
CTGAAGGACCTGGAATATGGCGAGA25540.11089723855454958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8850.012.3489394
CACCGCA701.092436E-412.2129737
CCGTCCA658.0332335E-411.69105159
AGTCGTC2600.011.32570658
TTAGACA2650.011.1170844
TGTAGGA28800.011.0864762
GCCGTGC600.00588181711.0821438
GGCGAGG11600.011.05100619
GTCTAGG1752.0008883E-1110.8743991
GGTATCA18100.010.8293391
GTAGGAC28250.010.5961553
CAAGTCG2550.010.4304786
AGGACGT29200.010.4145915
AGGACCT17100.010.3369375
CTGTAGG30950.010.3298011
AAGTCGT2600.010.2296717
GGACGTG28900.010.1565696
GACGTGG16200.010.1439717
TTAAGAC2250.010.1364273
GTACCGT750.002650282810.1324646
Produced by FastQC (version 0.11.5)