FastQCFastQC Report
Thu 26 May 2016
SRR1512493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247959
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT92050.28340874992572257No Hit
CTGTAGGACGTGGAATATGGCAAGA82860.2551140577821333No Hit
GTCCTAAAGTGTGTATTTCTCATTT82210.2531128009928697No Hit
CTTTAGGACGTGAAATATGGCGAGG70060.2157046933166336No Hit
GTATCAACGCAGAGTACTTTTTTTT55600.1711844268970144No Hit
GTCCTACAGTGTGCATTTCTCATTT52860.16274835981611838No Hit
GTACATGGGAAGCAGTGGTATCAAC38110.11733522498282767No Hit
CTGAAGGACCTGGAATATGGCGAGA34980.10769840382837345No Hit
CTGTAGGACCTGGAATATGGCGAGA34270.10551241564317776No Hit
GGTATCAACGCAGAGTACTTTTTTT32840.10110965070679771No Hit
TATCAACGCAGAGTACTTTTTTTTT32550.10021678229312624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG400.003987871614.8653971
TCCAACG3050.013.970918
ACGCGAA500.001555354513.2363688
TAGGACC15550.013.2268824
CCAACGA2600.013.11277319
TACACCG802.0534699E-613.0325945
GGACGCG701.1152074E-412.1863216
GGCGAGG23900.011.57045419
AGGACCT31600.010.8285
TGTAGGA55600.010.7397432
GTCCTAC47850.010.72835351
AATGTCC13900.010.6788798
GTCCTAA26950.010.6641061
TAGAAAT15850.010.5247454
GTTCCGG854.4693134E-410.4932221
TGGCGAG54150.010.47466218
TGTCCAC15300.010.47008810
AGGACGT54350.010.463575
GGACCTG31600.010.4380726
GGACGTG53650.010.4057586
Produced by FastQC (version 0.11.5)