FastQCFastQC Report
Thu 26 May 2016
SRR1512493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247959
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA101540.31262709904897196No Hit
GTCCTACAGTGGACATTTCTAAATT100340.3089324711303314No Hit
GTCCTAAAGTGTGTATTTCTCATTT88500.27247880899974414No Hit
CTTTAGGACGTGAAATATGGCGAGG84680.2607175767920716No Hit
GTATCAACGCAGAGTACTTTTTTTT74110.22817406254204564No Hit
GTCCTACAGTGTGCATTTCTCATTT56910.1752177290415304No Hit
TATCAACGCAGAGTACTTTTTTTTT50190.15452781269714302No Hit
GGTATCAACGCAGAGTACTTTTTTT46040.14175055781184429No Hit
GTACATGGGAAGCAGTGGTATCAAC44970.13845618125105644No Hit
CTGAAGGACCTGGAATATGGCGAGA44930.13833302698710176No Hit
CTGTAGGACCTGGAATATGGCGAGA42510.1308821940178432No Hit
ATTTAGAAATGTCCACTGTAGGACG37640.11588816238136011No Hit
TTTCTAAATTTTCCACCTTTTTCAG36150.11130066604904804No Hit
GTCCTTCAGTGTGCATTTCTCATTT33300.1025259247422766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3200.014.24463118
GACGTTA551.9631021E-413.8165917
CCAACGA2200.013.81255119
TAGGACC16950.013.4005494
CGTGCGC957.4074705E-812.99850310
GCGCCAC1550.012.86849613
GCGTGCG1103.8251528E-812.0895189
GGCGAGG22400.012.08212719
TAGAAAT14900.011.7361574
AGGACCT30850.011.2750975
AAATGTC14050.011.15537
GGACCTG29800.011.124736
GAAATGT14400.011.0822296
GGCGTGC1553.1468517E-1011.0309898
TGTAGGA56450.010.8920082
TACACCG700.001487273810.8614135
CACCTTT13750.010.844521514
GCCGGTT2200.010.79421211
CCGTTTC3000.010.76459913
AGGACGT55600.010.700255
Produced by FastQC (version 0.11.5)