FastQCFastQC Report
Thu 26 May 2016
SRR1512492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297824
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37500.2889451882535691No Hit
GTCCTACAGTGGACATTTCTAAATT29020.22360504968316194No Hit
CTGTAGGACGTGGAATATGGCAAGA26640.20526666173533545No Hit
GTCCTAAAGTGTGTATTTCTCATTT26510.2042649850827231No Hit
CTTTAGGACGTGAAATATGGCGAGG23900.1841543999802747No Hit
GGTATCAACGCAGAGTACTTTTTTT23550.18145757822324138No Hit
TATCAACGCAGAGTACTTTTTTTTT22320.17198017604852428No Hit
GTCCTACAGTGTGCATTTCTCATTT16700.12867692383558943No Hit
GTACATGGGAAGCAGTGGTATCAAC15520.11958478191187712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.013.1944484
GTCCTAT1454.5474735E-1112.3081211
ATGCCGG854.0872274E-612.25435119
ATAGGAC1750.011.9148253
GTATAGG1102.8543582E-711.717581
TCAGTAC1302.7102942E-911.6648653
GTGCAAG1052.0737498E-611.3312851
AATCCCG1105.177408E-711.19095719
GTATTAC906.161413E-511.0165271
CAGTACT1553.255991E-1011.0063644
GGCGAGG8550.010.96441919
AATGCCG951.4117102E-510.96272318
TATAGGA2350.010.8892762
GATATAC2550.010.8869221
GTCCTAC15800.010.6679031
TGTAGGA17550.010.6387882
GGACCTG9400.010.5867966
GTAGGAC17050.010.5060743
GTACTAA854.446114E-410.4981021
AGGACGT18950.010.40296655
Produced by FastQC (version 0.11.5)