FastQCFastQC Report
Thu 26 May 2016
SRR1512492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297824
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50090.3859537194565673No Hit
TATCAACGCAGAGTACTTTTTTTTT33400.25735384767117886No Hit
GGTATCAACGCAGAGTACTTTTTTT32950.25388650541213603No Hit
CTGTAGGACGTGGAATATGGCAAGA31900.24579604014103607No Hit
GTCCTACAGTGGACATTTCTAAATT30740.2368580023177257No Hit
GTCCTAAAGTGTGTATTTCTCATTT30450.23462349286189807No Hit
CTTTAGGACGTGAAATATGGCGAGG28920.22283452918115246No Hit
GTCCTACAGTGTGCATTTCTCATTT17920.13807727396010552No Hit
GTACATGGGAAGCAGTGGTATCAAC17430.1343017235002589No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16370.126134206178958No Hit
CTGAAGGACCTGGAATATGGCGAGA15120.11650269990383905No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13810.10640888132751436No Hit
CTGTAGGACCTGGAATATGGCGAGA13560.10448258007249057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCGG400.00529434314.24355719
GTACTAG550.00303687712.1073671
TAGGACC5550.011.6442474
CTAGGAC1800.011.6157073
TCTACAC1803.6379788E-1211.087723
GCCTAGA951.3392568E-511.0149731
CTACACT2250.010.9821224
GACGTGG9100.010.7516137
TGTAGGA18750.010.6442122
TAGGACA5200.010.60034754
AGGACGT16550.010.5661145
TATACAC4500.010.5597333
GGACGTG16500.010.5352526
GGCGAGA10200.010.3335619
CTGTAGG18800.010.2719421
GCCTAAG1303.766745E-710.2446951
ATAGGAC1951.4551915E-1110.2348183
GTATAGA1502.3905159E-810.1471261
GTAGGAC18550.010.1441753
CACCTTT4700.010.10375914
Produced by FastQC (version 0.11.5)