FastQCFastQC Report
Thu 26 May 2016
SRR1512491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258001
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38410.30532567144223255No Hit
GTACATGGGAAGCAGTGGTATCAAC33440.2658185486339041No Hit
GGTATCAACGCAGAGTACTTTTTTT24000.19077886265591204No Hit
CCCATGTACTCTGCGTTGATACCAC23180.1842605848485017No Hit
TATCAACGCAGAGTACTTTTTTTTT22020.17503960648679928No Hit
GTCCTACAGTGGACATTTCTAAATT19070.15158970461867677No Hit
CTGTAGGACGTGGAATATGGCAAGA17840.14181228790756129No Hit
GTCCTAAAGTGTGTATTTCTCATTT17400.13831467542553622No Hit
CTTTAGGACGTGAAATATGGCGAGG14250.11327494970194778No Hit
GAGTACATGGGAAGCAGTGGTATCA13480.10715412785840393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC456.8777084E-414.744463
GTATTAG759.708943E-613.1943331
TCTATAC1103.9322913E-812.063653
TAGGACC2950.011.8883914
TGTCGAG907.7152E-611.57568318
GTCGAGG752.132875E-411.36521619
GTATAGA1251.2740566E-711.0832391
GTATATA2250.010.9952761
GTTCTAG1001.5267047E-510.88532451
CAAGACT1752.1827873E-1110.8326654
GTTCTAA1209.008527E-710.7203941
GTTGTAT650.00786277610.6569611
GCCCTAA750.001885570710.5554671
GTCCTAC9800.010.5016121
ATAGGAC1909.094947E-1210.4763283
TGTAGGA11100.010.4183182
GGCGAGG5200.010.38168819
ATGTCGA1106.132317E-610.35061117
TTAGGAC7700.010.3402713
GGACGTG11850.010.3184576
Produced by FastQC (version 0.11.5)