FastQCFastQC Report
Thu 26 May 2016
SRR1512491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258001
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44480.35357682545562363No Hit
GTACATGGGAAGCAGTGGTATCAAC36000.28616829398386806No Hit
TATCAACGCAGAGTACTTTTTTTTT30750.2444354177778873No Hit
GGTATCAACGCAGAGTACTTTTTTT30310.24093780529586226No Hit
CCCATGTACTCTGCGTTGATACCAC28790.22885514399432114No Hit
CTGTAGGACGTGGAATATGGCAAGA22240.17678841272781182No Hit
GTCCTACAGTGGACATTTCTAAATT21850.17368825620965325No Hit
GTCCTAAAGTGTGTATTTCTCATTT18970.1507947926909438No Hit
CTTTAGGACGTGAAATATGGCGAGG18310.1455483739679062No Hit
GAGTACATGGGAAGCAGTGGTATCA16320.1297296266060202No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14390.11438782640077394No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13420.10667718070176416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2050.013.4587671
ATCTAGG905.3068834E-712.6852741
TAATACC604.084351E-412.6691314
GGCGAGG5050.012.60040119
TAGGACC3450.011.8428854
CTACACT1850.011.813114
GTCCTAG1604.3655746E-1111.2978221
TGGCGAG10850.011.11667518
GTCCTAT1303.1926902E-810.9776411
TTAATAC1053.4558252E-610.8592563
TGTACCG803.7600574E-410.6904315
TAGGACA3750.010.6420714
TGTAGGA12650.010.5158812
CTGTAGG12350.010.4769061
GTAGGAC12200.010.3585743
GTCCTAA6450.010.1777211
GGACCTG7900.010.10001856
AGGACCT7950.010.0404885
TATACAC4300.09.9437953
GTATTAC1451.5807927E-79.8420241
Produced by FastQC (version 0.11.5)