FastQCFastQC Report
Thu 26 May 2016
SRR1512490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506733
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT106610.30401516169038245No Hit
CTGTAGGACGTGGAATATGGCAAGA95140.27130665494065276No Hit
GTCCTAAAGTGTGTATTTCTCATTT91210.26009964260181767No Hit
CTTTAGGACGTGAAATATGGCGAGG72480.20668810542462174No Hit
GTACATGGGAAGCAGTGGTATCAAC64660.18438814703029857No Hit
GTCCTACAGTGTGCATTTCTCATTT56860.1621452217776489No Hit
GTATCAACGCAGAGTACTTTTTTTT56350.1606908766649756No Hit
CCCATGTACTCTGCGTTGATACCAC47740.1361381091745508No Hit
CTGAAGGACCTGGAATATGGCGAGA40630.11586282730963549No Hit
GTCCTTCAGTGTGCATTTCTCATTT37640.10733637262945311No Hit
CTGTAGGACCTGGAATATGGCGAGA36450.1039429006998822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17900.012.9188644
CACGTAA600.00432557911.5488451
GGCGAGG23750.011.28785119
AGGACCT33450.011.1065065
AGGACGT59400.011.0409685
TGTAGGA60050.011.0320932
ACCTTTT13550.010.92404215
CCACCTT13250.010.88071813
GGACCTG32350.010.8689386
GGACGTG58400.010.8567746
GTAGGAC59450.010.8563233
ATTTAGA13200.010.7989221
GTCCTAC50950.010.7635961
GTCCTAA25800.010.7431131
TAGAAAT14150.010.6494754
GACGTGG29550.010.55178457
TACACCG1355.8978912E-810.5304035
TTTAGAA16000.010.4844832
TGCGTGA1002.4585288E-510.42748910
AATGTCC13150.010.426878
Produced by FastQC (version 0.11.5)