FastQCFastQC Report
Thu 26 May 2016
SRR1512490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506733
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA117060.33381497821476575No Hit
GTCCTACAGTGGACATTTCTAAATT116390.33190436796870476No Hit
GTCCTAAAGTGTGTATTTCTCATTT98820.2818007530085695No Hit
CTTTAGGACGTGAAATATGGCGAGG93620.2669721361734697No Hit
GTACATGGGAAGCAGTGGTATCAAC75470.2152145601048041No Hit
GTATCAACGCAGAGTACTTTTTTTT68360.19493927823988882No Hit
GTCCTACAGTGTGCATTTCTCATTT61680.17589020892095292No Hit
CCCATGTACTCTGCGTTGATACCAC57500.16397028231119964No Hit
CTGAAGGACCTGGAATATGGCGAGA54800.15627080818528244No Hit
CTGTAGGACCTGGAATATGGCGAGA46760.1333434852325512No Hit
TATCAACGCAGAGTACTTTTTTTTT46310.13206023954489834No Hit
GGTATCAACGCAGAGTACTTTTTTT43940.12530181225659326No Hit
ATTTAGAAATGTCCACTGTAGGACG39820.11355298507186033No Hit
GTCCTTCAGTGTGCATTTCTCATTT39330.11215567310086055No Hit
TTTCTAAATTTTCCACCTTTTTCAG38400.10950363201304462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGT400.005281404614.2497766
TAGGACC18600.013.639414
GGCGAGG22600.011.51406919
CCACCTT14500.011.33333413
TGTAGGA60450.011.2848152
TTTAGAA16950.011.2665962
AGGACCT36050.011.2284615
GACGGGA1104.975409E-711.2270967
GCGCCAC1950.011.20399213
TAGAAAT14950.011.0587334
CTGTAGG59650.010.9838131
ACCTTTT15000.010.95493215
ATAGGAC6350.010.9223343
GGACCTG34650.010.9118046
GTAGGAC60300.010.9032283
GACGTGG28550.010.8807397
TACACCG700.001489569210.8594495
CACCTTT15100.010.81947814
AGGACGT58500.010.6390125
ATTTAGA14850.010.6298231
Produced by FastQC (version 0.11.5)