FastQCFastQC Report
Thu 26 May 2016
SRR1512489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052110
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47490.23142034296407113No Hit
GTCCTACAGTGGACATTTCTAAATT45700.22269761367568014No Hit
GTCCTAAAGTGTGTATTTCTCATTT42080.20505723377401797No Hit
CTGTAGGACGTGGAATATGGCAAGA40580.1977476840910088No Hit
CTTTAGGACGTGAAATATGGCGAGG35520.17309013649365773No Hit
TATCAACGCAGAGTACTTTTTTTTT28900.14083065722597718No Hit
GGTATCAACGCAGAGTACTTTTTTT28570.13922255629571514No Hit
GTCCTACAGTGTGCATTTCTCATTT26170.12752727680290044No Hit
GTACATGGGAAGCAGTGGTATCAAC21580.1051600547728923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.013.5054244
CCAACGA1001.049375E-813.26166919
TCCAACG1251.4788384E-912.12376818
GGCGAGG12750.012.11009519
GTCCTAG3300.011.69780351
ACTGCGC658.333993E-411.6398128
CGTGCGC752.1162943E-411.37549410
GGACGTG27850.010.9237866
GTATAAC1457.610652E-910.9220871
GACGTGA15100.010.9210827
GTCCTAT3000.010.8879561
AGGACGT28400.010.8457195
ATAGGAC3500.010.8314183
TTAGGAC17550.010.8005583
ACGTGAA16700.010.7032268
TAGGCTG1605.675247E-1010.6621765
GTGTAGG2750.010.438041
CACCTTT6550.010.429749514
TGGCGAG26400.010.40451118
TGTAGGA29600.010.3419912
Produced by FastQC (version 0.11.5)