FastQCFastQC Report
Thu 26 May 2016
SRR1512489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052110
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60620.2954032678560116No Hit
GTCCTACAGTGGACATTTCTAAATT51440.2506688237959953No Hit
CTGTAGGACGTGGAATATGGCAAGA50270.24496737504324817No Hit
GTCCTAAAGTGTGTATTTCTCATTT45920.22376968096252148No Hit
CTTTAGGACGTGAAATATGGCGAGG43830.2135850417375287No Hit
TATCAACGCAGAGTACTTTTTTTTT41150.20052531297055226No Hit
GGTATCAACGCAGAGTACTTTTTTT39730.19360560593730355No Hit
GTCCTACAGTGTGCATTTCTCATTT28680.1397585899391358No Hit
GTACATGGGAAGCAGTGGTATCAAC24210.11797613188376842No Hit
CTGAAGGACCTGGAATATGGCGAGA22130.10784022298999567No Hit
CTGTAGGACCTGGAATATGGCGAGA20940.10204131357480838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA759.710984E-713.929008519
TAGGACC8650.012.5233644
CGGTTTC701.0924054E-412.21287613
GGCGAGG12100.011.53774119
TGAGCCG600.005865812311.0861065
AACTGCG600.005881797511.0820537
AGGACCT17750.010.92104155
ATAGGAC2100.010.859333
TGGCGAG26650.010.61985118
CTAGGAC2800.010.5199773
AATGTCC7550.010.4425038
GTCCTAC24150.010.438491
GGACCTG17650.010.386656
GAAATGT7900.010.3403276
TTTAGAA8750.010.3163632
AGGACGT28100.010.3139685
TCGAACT1201.5302012E-610.28847219
TAGGACA8500.010.2849254
GTACTAG1303.7720383E-710.2445671
GGACGTG27300.010.2293396
Produced by FastQC (version 0.11.5)