FastQCFastQC Report
Thu 26 May 2016
SRR1512488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69070.30888890777114764No Hit
CTGTAGGACGTGGAATATGGCAAGA64350.2877805301154387No Hit
GTCCTAAAGTGTGTATTTCTCATTT63810.2853655886039805No Hit
GTATCAACGCAGAGTACTTTTTTTT54330.24296994873615826No Hit
CTTTAGGACGTGAAATATGGCGAGG52110.23304185585571888No Hit
GTCCTACAGTGTGCATTTCTCATTT39490.1766037783101581No Hit
TATCAACGCAGAGTACTTTTTTTTT33660.15053135421422947No Hit
GGTATCAACGCAGAGTACTTTTTTT32690.14619340372142486No Hit
GTACATGGGAAGCAGTGGTATCAAC28060.1254875163176256No Hit
CTGAAGGACCTGGAATATGGCGAGA25960.11609607710639919No Hit
CTGTAGGACCTGGAATATGGCGAGA25160.11251838597831293No Hit
GTCCTTCAGTGTGCATTTCTCATTT24090.10773322409449756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12200.014.1396314
ATGTCGA1650.012.65354217
AATGTCC8750.012.4283178
GGCGAGG16500.012.00007519
TATGTCG1900.011.98675816
TGTCGAG1850.011.77631718
AGGACCT23000.011.4562765
AAATGTC9400.011.2931927
GTCCTAG3700.011.2194071
ACCTTTT9000.011.07135115
CTGGTCG600.005987292611.055689
ACCGCCC600.00608202511.0323828
TAGAAAT11000.011.0291954
ATGTCCA9550.011.0143369
TGTAGGA42100.011.0093842
TTAGGAC24350.010.9378993
AGGACGT40700.010.8987835
GGACGTG40050.010.8626736
GAAATGT10150.010.8322456
CACCTTT8850.010.72285814
Produced by FastQC (version 0.11.5)