FastQCFastQC Report
Thu 26 May 2016
SRR1512488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA77690.3474385296762771No Hit
GTCCTACAGTGGACATTTCTAAATT77650.3472596451198728No Hit
GTATCAACGCAGAGTACTTTTTTTT69640.31143801269990906No Hit
GTCCTAAAGTGTGTATTTCTCATTT69190.30942556144036054No Hit
CTTTAGGACGTGAAATATGGCGAGG64250.28733331872442786No Hit
TATCAACGCAGAGTACTTTTTTTTT48420.21653975552742097No Hit
GGTATCAACGCAGAGTACTTTTTTT45060.2015134527894587No Hit
GTCCTACAGTGTGCATTTCTCATTT42200.18872320700655032No Hit
CTGAAGGACCTGGAATATGGCGAGA34590.15469042015062973No Hit
GTACATGGGAAGCAGTGGTATCAAC33500.1498158159886122No Hit
CTGTAGGACCTGGAATATGGCGAGA31390.1403796556382847No Hit
TTTCTAAATTTTCCACCTTTTTCAG26750.11962904709538437No Hit
ATTTAGAAATGTCCACTGTAGGACG26420.1181532495050488No Hit
GAATATGGCAAGAAAACTGAAAATC26300.11761659583583585No Hit
GTCCTTCAGTGTGCATTTCTCATTT25330.11327864534303125No Hit
CCCATGTACTCTGCGTTGATACCAC23560.1053630037221404No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23170.10361887929719836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13400.013.40114
AGGACCT25700.011.4245195
TCCAACG1104.9941264E-711.22329518
GGCGAGG17100.011.21749419
GGACCTG25000.011.0955436
TGTAGGA43150.010.9433252
TTCCACC9600.010.88521211
GTAGGAC41400.010.717423
CTGTAGG41900.010.7165721
ACCTTTT9950.010.69159215
GTATAAG2350.010.5252731
AAGTCGT2650.010.3957987
GTCCTAA19950.010.2523511
TGTCGAG1951.4551915E-1110.22714518
CCACCTT10100.010.15754213
ACGTGAT750.002649254210.13291558
GGACGTG38450.010.12962156
AGGACGT39150.010.1208485
GGTGTAC856.5978494E-410.058418
GACCTGG26450.010.0562967
Produced by FastQC (version 0.11.5)