FastQCFastQC Report
Thu 26 May 2016
SRR1512487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118824
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83530.3942281189943101No Hit
CCCATGTACTCTGCGTTGATACCAC60690.28643247386285975No Hit
GTATCAACGCAGAGTACTTTTTTTT53450.2522625758439587No Hit
GTCCTACAGTGGACATTTCTAAATT39160.18481950364919408No Hit
GTCCTAAAGTGTGTATTTCTCATTT36790.17363405360709527No Hit
CTGTAGGACGTGGAATATGGCAAGA36510.17231256583840848No Hit
GAGTACATGGGAAGCAGTGGTATCA35230.16627147889584035No Hit
GGTATCAACGCAGAGTACTTTTTTT33830.15966404005240642No Hit
TATCAACGCAGAGTACTTTTTTTTT31440.14838419802682998No Hit
CTTTAGGACGTGAAATATGGCGAGG30550.14418375476207557No Hit
CATGTACTCTGCGTTGATACCACTG21960.10364239785843468No Hit
GTATCAACGCAGAGTACATGGGAAG21620.10203773413931501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG508.882968E-515.1662774
TAGGACC8550.012.9711584
AAGCCCG658.1764214E-411.6663685
AAGTCGT1800.011.585357
TATTCCG700.001519679410.83305555
AGTCGTC2100.010.8087118
CAAGTCG1951.8189894E-1210.694176
TGGACGG803.8500773E-410.6637895
GTATTAG1501.31294655E-810.5469421
GTGTAGG4050.010.4981141
GGCGAGG9700.010.448719
GGACGTG21150.010.3976836
AGGACGT21400.010.3648045
TGTAGGA24550.010.3476642
GGACCTG16050.010.3352756
GTAGGAC24350.010.3158713
ATTTAGA5100.010.2755141
AGGACCT16850.010.2383615
GTCCTAC20400.010.2270441
ATAGGAC2550.010.0365083
Produced by FastQC (version 0.11.5)