FastQCFastQC Report
Thu 26 May 2016
SRR1512487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118824
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC93820.44279279449354925No Hit
CCCATGTACTCTGCGTTGATACCAC73810.3483536150241832No Hit
GTATCAACGCAGAGTACTTTTTTTT62350.29426700849150284No Hit
CTGTAGGACGTGGAATATGGCAAGA45610.21526091832072886No Hit
TATCAACGCAGAGTACTTTTTTTTT42560.20086614084039073No Hit
GAGTACATGGGAAGCAGTGGTATCA41600.1963353256334646No Hit
GTCCTACAGTGGACATTTCTAAATT41060.19378674207956867No Hit
GGTATCAACGCAGAGTACTTTTTTT40760.19237086232740425No Hit
GTCCTAAAGTGTGTATTTCTCATTT39250.1852442675748434No Hit
CTTTAGGACGTGAAATATGGCGAGG36960.1744363854666551No Hit
CATGTACTCTGCGTTGATACCACTG26890.1269100217856698No Hit
TATCAACGCAGAGTACATGGGAAGC23860.11260963628880927No Hit
GTATCAACGCAGAGTACATGGGAAG23840.11251524430533164No Hit
GCGTTGATACCACTGCTTCCCATGT23200.10949470083404757No Hit
ACGCAGAGTACATGGGAAGCAGTGG22170.10463351368494977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG456.780154E-414.77306118
ATCGCGA400.005290308514.24578717
TATCGCG500.001503206313.29606816
ATATCGC655.46583E-513.15088915
TAGGACC8000.012.7090924
CTCGAAC1501.8189894E-1212.66262318
TCGTGCA701.09186876E-412.2135569
GGACCGT550.003069363812.0907586
TCGAACT1603.6379788E-1211.87092919
ACTGCGC658.029547E-411.6916118
ACCGTCC658.029547E-411.6916118
AGGACCT16300.011.4264085
GGCGAGG9650.011.12053919
TCTTACA3450.011.0166972
CCGCCTT700.001492531910.8564959
GGACCTG15150.010.7853456
CCTCGAA1951.8189894E-1210.7147817
CTAGACT1605.4205884E-1010.6898914
TGTAGGA24600.010.5834192
AGGACGT23250.010.503925
Produced by FastQC (version 0.11.5)