FastQCFastQC Report
Thu 26 May 2016
SRR1512486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315814
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61690.2663858150956856No Hit
GTCCTAAAGTGTGTATTTCTCATTT58150.2510996133540949No Hit
CTGTAGGACGTGGAATATGGCAAGA56820.24535649236078544No Hit
GTACATGGGAAGCAGTGGTATCAAC54100.2336111622090548No Hit
GTATCAACGCAGAGTACTTTTTTTT49640.21435227526908465No Hit
CTTTAGGACGTGAAATATGGCGAGG49200.21245229539159882No Hit
CCCATGTACTCTGCGTTGATACCAC37920.1637437203505981No Hit
GTCCTACAGTGTGCATTTCTCATTT32180.13895761922157823No Hit
GGTATCAACGCAGAGTACTTTTTTT30030.12967362663840878No Hit
TATCAACGCAGAGTACTTTTTTTTT28340.12237597665442908No Hit
CTGTAGGACCTGGAATATGGCGAGA25320.10933520567713986No Hit
CTGAAGGACCTGGAATATGGCGAGA25280.10916248023373207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC552.0147706E-413.77401318
TAGGACC11050.013.5526294
TCCAACG1800.012.62617818
GTGTAGG4300.012.1974521
CGGATAC550.00312181712.0635033
TAAGACG1052.8039722E-711.7349924
GGCGAGG14550.011.19555719
GGACCTG21950.010.9250686
AGGACCT23200.010.7447555
TGTAGGA37800.010.7324672
TGGCGAG35300.010.64998418
GTCCTAC34700.010.4664221
CCAACGA1451.515582E-810.45038419
CACCTTT9000.010.435369514
GTAGGAC36700.010.4340633
TCCTACA39400.010.27257252
CGCCACC1303.9179758E-710.21644714
ATAGGAC4000.010.1893533
GTCCTAA19450.010.1758821
GTTTAGG4700.010.10661
Produced by FastQC (version 0.11.5)