FastQCFastQC Report
Thu 26 May 2016
SRR1512486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315814
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66750.28823558368677277No Hit
CTGTAGGACGTGGAATATGGCAAGA66750.28823558368677277No Hit
GTCCTAAAGTGTGTATTTCTCATTT64120.27687888578270964No Hit
GTACATGGGAAGCAGTGGTATCAAC62560.2701425934898053No Hit
GTATCAACGCAGAGTACTTTTTTTT60420.26090178226748784No Hit
CTTTAGGACGTGAAATATGGCGAGG59330.2561950139346251No Hit
CCCATGTACTCTGCGTTGATACCAC45610.19695018684574842No Hit
TATCAACGCAGAGTACTTTTTTTTT39270.1695732040656115No Hit
GGTATCAACGCAGAGTACTTTTTTT39190.16922775317879588No Hit
GTCCTACAGTGTGCATTTCTCATTT35020.15122112570353233No Hit
CTGAAGGACCTGGAATATGGCGAGA33170.14323257394592137No Hit
CTGTAGGACCTGGAATATGGCGAGA30940.13360313047593633No Hit
GAGTACATGGGAAGCAGTGGTATCA27300.11788511512582617No Hit
TTTCTAAATTTTCCACCTTTTTCAG26940.11633058613515594No Hit
ATTTAGAAATGTCCACTGTAGGACG26240.11330789087551937No Hit
GAATATGGCAAGAAAACTGAAAATC24110.10411026101405381No Hit
GTCCTTCAGTGTGCATTTCTCATTT23970.1035057219621265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT400.00528196914.24925412
TTGGCGA500.001503458713.29585817
TAGGACC12350.013.1571384
GGCGAGG15650.012.74253519
GCGTGCG658.0295466E-411.6916969
CGTGCGC600.005879420311.08275310
GGACCTG23400.010.8800086
ATACGGC700.001493606610.85563815
AGGACCT23750.010.7626925
AATGTCC8850.010.6263338
CACCTTT7650.010.554090514
AGGACGT39750.010.4466375
CTAGGAC3950.010.3434653
TGGCGAG38600.010.33312218
TGTAGGA40250.010.3159742
GGACGTG39250.010.3105126
TCCAACG2151.8189894E-1210.15918718
GACGTGA21100.010.0845627
TAGAAAT10750.010.0769394
AAATGTC9050.010.0766047
Produced by FastQC (version 0.11.5)