FastQCFastQC Report
Thu 26 May 2016
SRR1512485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579807
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46230.2926306821023074No Hit
GTACATGGGAAGCAGTGGTATCAAC44570.28212306946354837No Hit
GTCCTACAGTGGACATTTCTAAATT34990.22148275074107154No Hit
CCCATGTACTCTGCGTTGATACCAC32910.20831658550696386No Hit
CTGTAGGACGTGGAATATGGCAAGA31950.20223989386045255No Hit
GTCCTAAAGTGTGTATTTCTCATTT30720.19445413268836004No Hit
GGTATCAACGCAGAGTACTTTTTTT28750.1819842550387484No Hit
TATCAACGCAGAGTACTTTTTTTTT27330.17299581531161717No Hit
CTTTAGGACGTGAAATATGGCGAGG25750.16299459364340074No Hit
GAGTACATGGGAAGCAGTGGTATCA18810.11906517694882982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG500.001525227113.2700774
TAGGACC5800.013.2373914
TATTAGG1407.057679E-1011.509762
ATTAGAC855.446226E-511.1513243
GTGTAGG2800.010.9388451
GTCCTAA9000.010.758491
TGTAGGA18900.010.7324122
AATCCCG1605.711627E-1010.65769219
CTAGGAC2050.010.6345563
GGCGAGG8350.010.55132819
TAGTACT1451.4941179E-810.4591734
GTAGGAC18750.010.3127453
ACTGTTC3400.010.2901018
AGGACCT11400.010.2269385
GACGTGG9350.010.13756757
CTAATCC1151.05399795E-59.890743
GTATTAG2001.2551027E-109.8802461
AGGACGT20200.09.8540175
CTGTAGG16550.09.8503961
GGACCTG11200.09.8171486
Produced by FastQC (version 0.11.5)