FastQCFastQC Report
Thu 26 May 2016
SRR1512485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579807
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54480.3448522509395135No Hit
GTACATGGGAAGCAGTGGTATCAAC51820.3280147511689719No Hit
CTGTAGGACGTGGAATATGGCAAGA39240.2483847710511474No Hit
CCCATGTACTCTGCGTTGATACCAC39210.2481948744371939No Hit
GTCCTACAGTGGACATTTCTAAATT38340.24268787263254307No Hit
TATCAACGCAGAGTACTTTTTTTTT36390.2303445927255671No Hit
GGTATCAACGCAGAGTACTTTTTTT36370.23021799498293147No Hit
GTCCTAAAGTGTGTATTTCTCATTT34130.21603904780773853No Hit
CTTTAGGACGTGAAATATGGCGAGG33060.2092660685767312No Hit
GAGTACATGGGAAGCAGTGGTATCA21910.13868782705735574No Hit
CTGAAGGACCTGGAATATGGCGAGA16860.10672189704185385No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16790.10627880494262906No Hit
GTCCTACAGTGTGCATTTCTCATTT16780.10621550607131125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC250.006039263718.99610715
GGACCGC456.766431E-414.7766216
CGGTGAA400.005289668714.24572716
TGTACCG852.6923226E-713.4145375
GTCTAGG1207.203198E-1012.6893761
TGGACGG550.003063737412.0934085
TAGGACC6100.011.6827424
ACAGCGC752.0753652E-411.3991078
TCTATAC1201.2730743E-711.0845713
CTGTAGG19350.011.0171331
TGTAGGA20100.011.0136672
GTATTAA1657.8216544E-1110.9590071
TGGACCG700.001488856610.8593865
CCAGTAC1502.1282176E-910.7678693
GTAGGAC20250.010.6505723
TCCAACG1106.065922E-610.36052918
AGGACCT11850.010.3439095
AGGACGT20400.010.2937945
TAGACTG1951.4551915E-1110.2328835
GTCCTAA10300.010.1638211
Produced by FastQC (version 0.11.5)