FastQCFastQC Report
Thu 26 May 2016
SRR1512484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856649
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55140.29698666791622974No Hit
GTCCTAAAGTGTGTATTTCTCATTT55000.2962326212439723No Hit
CTGTAGGACGTGGAATATGGCAAGA51770.2788356873054627No Hit
GTACATGGGAAGCAGTGGTATCAAC51390.2767889891950498No Hit
GTATCAACGCAGAGTACTTTTTTTT47410.25535251951230414No Hit
CTTTAGGACGTGAAATATGGCGAGG45130.24307233084982674No Hit
CCCATGTACTCTGCGTTGATACCAC38730.20860162583234634No Hit
GTCCTACAGTGTGCATTTCTCATTT30040.1617968716757987No Hit
GGTATCAACGCAGAGTACTTTTTTT28610.15409482352345544No Hit
TATCAACGCAGAGTACTTTTTTTTT26440.142407100103466No Hit
CTGAAGGACCTGGAATATGGCGAGA23080.12430997996928875No Hit
ATTTAGAAATGTCCACTGTAGGACG22140.11924709516984633No Hit
GTCCTTCAGTGTGCATTTCTCATTT21830.11757742039556211No Hit
CTGTAGGACCTGGAATATGGCGAGA21540.11601546657445753No Hit
GAGTACATGGGAAGCAGTGGTATCA21510.11585388514468808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG4150.013.838651
TCCAACG1650.013.20342818
GTGCGCC802.0510452E-613.03319711
TAGGACC9600.012.8346834
CCGGTTT1201.0342774E-811.84932412
CCAACGA1201.0435542E-811.84227319
CGGTTTC1302.6902853E-911.67144913
GGCGAGG14350.011.61945619
GTCCTAT1801.8189894E-1211.5520921
GCCGGTT1157.2994226E-811.53927311
CTAGGAC2850.011.3069923
ACACCGT1105.1212373E-711.2011796
GTGTAGA3100.011.1794421
CCCGCAC600.005979020611.0575743
GGGACGG600.00599831211.05278819
AATGTCC8650.010.9325798
GTCCTAA18050.010.91665651
CCTACAC2800.010.8319093
TAAGACG700.001520866810.8319094
GGACGTG34050.010.7722616
Produced by FastQC (version 0.11.5)