FastQCFastQC Report
Thu 26 May 2016
SRR1512484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856649
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA61390.33064946578486293No Hit
GTACATGGGAAGCAGTGGTATCAAC60540.3260713252747288No Hit
GTCCTACAGTGGACATTTCTAAATT60440.3255327205088307No Hit
GTCCTAAAGTGTGTATTTCTCATTT60360.32510183669611215No Hit
GTATCAACGCAGAGTACTTTTTTTT60110.32375532478136687No Hit
CTTTAGGACGTGAAATATGGCGAGG56050.3018879712859027No Hit
CCCATGTACTCTGCGTTGATACCAC45040.24258758656051843No Hit
GGTATCAACGCAGAGTACTTTTTTT39630.21344906872542954No Hit
TATCAACGCAGAGTACTTTTTTTTT39110.21064832394275926No Hit
GTCCTACAGTGTGCATTTCTCATTT32360.17429250224463536No Hit
CTGAAGGACCTGGAATATGGCGAGA31090.16745222171772908No Hit
GAGTACATGGGAAGCAGTGGTATCA26660.1435920305884419No Hit
ATTTAGAAATGTCCACTGTAGGACG26130.14073742532918176No Hit
CTGTAGGACCTGGAATATGGCGAGA25870.13933705293784662No Hit
TTTCTAAATTTTCCACCTTTTTCAG24550.1322274700279913No Hit
GAATATGGCAAGAAAACTGAAAATC23290.12544104997767483No Hit
GTCCTTCAGTGTGCATTTCTCATTT22710.12231714233546567No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20300.1093367674773207No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19830.10680532507759949No Hit
GGAATATGGCGAGAAAACTGAAAAT18670.10055750979318116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.002166222516.2904265
TCCAACG1200.015.03669918
CGTGCGC400.00528363914.248363510
CCAACGA1207.4396667E-1012.66248319
TAGGACC9300.012.4659714
TATAGCG658.0052577E-411.6956915
CTTAGAC1800.011.6141583
ATTAGGC600.005864920611.0862433
TAGAACG951.3561217E-511.0031834
AATGTCC8800.010.9021578
TCGAACT1053.4805398E-610.85355719
TAGAAAT9150.010.8009394
GTCCTAC32850.010.54161551
CCACCTT8500.010.50410913
GGCGAGG14400.010.42016819
GGACGTG31300.010.4096286
GTCCTAA16400.010.38365651
AAATGTC8700.010.3723737
AGGACGT32150.010.315585
GAAATGT9350.010.261136
Produced by FastQC (version 0.11.5)