FastQCFastQC Report
Thu 26 May 2016
SRR1512483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595527
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50610.1949893027504626No Hit
GTCCTACAGTGGACATTTCTAAATT42490.1637047119910523No Hit
CTGTAGGACGTGGAATATGGCAAGA39170.15091347537513577No Hit
GTCCTAAAGTGTGTATTTCTCATTT39080.15056672498494525No Hit
CTTTAGGACGTGAAATATGGCGAGG33870.13049373017502802No Hit
TATCAACGCAGAGTACTTTTTTTTT30130.11608432507155579No Hit
GGTATCAACGCAGAGTACTTTTTTT29600.11404235055154502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGC350.002236484316.2131028
TAGGACC7800.012.151944
GGCGAGG11750.011.68919719
GGTATCA14000.011.0921771
GTGTAGG4800.010.7153741
TAGGACT4700.010.6885374
AGGACGT24050.010.4835115
GGACGTG23600.010.44243056
TTAGGAC16900.010.4319733
AGGACCT15650.010.4172795
TGGCGAG26850.010.33560418
GTAGGAC24800.010.2811313
GTCCTAA13050.010.2321871
CCAACGA1401.011922E-710.1488719
GGACCTG15950.010.1024916
TGTAGGA26100.010.096282
TCCAACG2350.010.07591918
TAGGACG27450.09.841084
GACGTGA13050.09.8055497
CGAAATC1752.6466296E-99.75612713
Produced by FastQC (version 0.11.5)