FastQCFastQC Report
Thu 26 May 2016
SRR1512483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595527
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59720.23008814780196854No Hit
CTGTAGGACGTGGAATATGGCAAGA48980.18870926790590117No Hit
GTCCTACAGTGGACATTTCTAAATT46000.17722797720848213No Hit
CTTTAGGACGTGAAATATGGCGAGG42680.1644367405925656No Hit
GTCCTAAAGTGTGTATTTCTCATTT42100.16220212696689343No Hit
TATCAACGCAGAGTACTTTTTTTTT39850.15353336721213073No Hit
GGTATCAACGCAGAGTACTTTTTTT39590.15253164386269147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9350.013.3134414
TAGGTCG604.084499E-412.6700345
TCCAACG1850.012.3218
ACCGCTC550.003070820612.0901618
GGTATCA17200.011.3366111
GTATTGG2950.011.2850591
ACCGAGC600.005880162611.0826478
TGTAGGA27900.010.6597182
GTAGGAC26850.010.4041973
CTGTAGG27550.010.3920851
AGGACGT26200.010.30044655
GGCGAGG10700.010.29525519
AGGACCT18000.010.2944025
GTCCTAA14400.010.1722271
GGACGTG25800.010.1253426
TGGCGAG25400.010.05749418
GTCCTAC23250.010.0230731
CCACCTT7250.09.95762113
TCTACAC3950.09.862623
CCAACGT1356.4796586E-79.848206519
Produced by FastQC (version 0.11.5)