FastQCFastQC Report
Thu 26 May 2016
SRR1512482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432095
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54660.22474451039124704No Hit
GGTATCAACGCAGAGTACTTTTTTT34790.1430453991312017No Hit
TATCAACGCAGAGTACTTTTTTTTT33530.1378646804503936No Hit
GTCCTACAGTGGACATTTCTAAATT28010.11516819861066283No Hit
GTCCTAAAGTGTGTATTTCTCATTT27820.11438697912704891No Hit
CTGTAGGACGTGGAATATGGCAAGA26700.10978189585521947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6850.013.2837334
GTAGGAC19350.011.1684623
CCTTACG600.00597721611.0582482
CTAGTAC1201.3168756E-711.0582483
ATACGAT600.00597721611.0582486
GGACGTG18450.011.04540356
AGGACGT18800.011.0414425
AGGACCT12450.010.9630815
TTAGGAC12500.010.91922953
TGTAGGA20850.010.9105012
TCGAACT1658.367351E-1110.91012719
GTATAAG1654.765752E-1010.7690461
GACGTGG9400.010.68851957
GGCGAGG9150.010.66537919
TAGGACG20100.010.6574154
GTCCTAC16000.010.6119971
GGTATCA14250.010.5990091
TGGCGAG18500.010.54942318
GACGTGA10200.010.4077637
GGACCTG12150.010.3756396
Produced by FastQC (version 0.11.5)