FastQCFastQC Report
Thu 26 May 2016
SRR1512482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432095
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63210.2598993871538735No Hit
TATCAACGCAGAGTACTTTTTTTTT44470.18284647598058465No Hit
GGTATCAACGCAGAGTACTTTTTTT43560.17910484582222322No Hit
CTGTAGGACGTGGAATATGGCAAGA31520.12960020065005684No Hit
GTCCTAAAGTGTGTATTTCTCATTT31300.12869563072166176No Hit
GTCCTACAGTGGACATTTCTAAATT30430.12511846782300856No Hit
CTTTAGGACGTGAAATATGGCGAGG29010.11927988010336768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6650.011.4312944
AGGACGT17750.011.081745
GGACGTG17100.010.8881236
ACTGCGC700.001492802510.8563798
GACGTGG8650.010.7622497
GGTATCA18350.010.4221961
GTAGGAC19750.010.2954873
TATACTG3450.010.1910435
TGTAGAA7450.010.07560352
TGTAGGA21450.010.05538752
TTAGACC1054.0988423E-59.9541383
GTCCTAC16700.09.856641
GACGTGA9700.09.7931257
TAGGACT3600.09.7662144
TTAGGAC11400.09.7517213
ACGTGGA9900.09.69123658
TAGGACG19750.09.6706574
CTACCGT800.00450812849.5022644
CGTGGAA9800.09.4993319
ACTGCGT900.00111470669.4993318
Produced by FastQC (version 0.11.5)