FastQCFastQC Report
Thu 26 May 2016
SRR1512481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189465
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80200.25145282986331563No Hit
GTCCTAAAGTGTGTATTTCTCATTT72310.22671513874583982No Hit
CTGTAGGACGTGGAATATGGCAAGA70490.22100885258185934No Hit
CTTTAGGACGTGAAATATGGCGAGG60790.19059622852108427No Hit
GTATCAACGCAGAGTACTTTTTTTT55320.1734460168084616No Hit
GTCCTACAGTGTGCATTTCTCATTT51870.1626291556734437No Hit
CTGTAGGACCTGGAATATGGCGAGA34020.10666365675748125No Hit
TATCAACGCAGAGTACTTTTTTTTT34000.10660095031611884No Hit
GGTATCAACGCAGAGTACTTTTTTT33120.10384186689617224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC18450.013.6136084
TCCAACG2450.013.13990418
AACCGCT701.1153017E-412.1861977
AGGACCT31850.011.6059025
GTGTAGG6750.011.4450251
GGACCTG30100.011.3045096
CTAGGAC5350.011.161193
GGCGAGG20000.011.12719119
GTCCTAT4900.011.11712551
TGTAGGA52000.010.9727852
CCAACGA1658.54925E-1110.90479119
GGACGTG44900.010.871386
GTACCGT700.001521074910.8321756
CTACGGT700.001521074910.8321754
AGGACGT45950.010.7467215
TAGAAAT14300.010.7374894
GTAGGAC51100.010.6652413
CACCTTT11300.010.6620114
GACGTGA23300.010.6578387
CCGGTTT1701.4370016E-1010.59673612
Produced by FastQC (version 0.11.5)