FastQCFastQC Report
Thu 26 May 2016
SRR1512481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189465
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA87920.27565751622921086No Hit
GTCCTACAGTGGACATTTCTAAATT84580.26518554052168625No Hit
GTCCTAAAGTGTGTATTTCTCATTT80330.2518604217321714No Hit
CTTTAGGACGTGAAATATGGCGAGG74800.23452209069546145No Hit
GTATCAACGCAGAGTACTTTTTTTT69810.21887683357553694No Hit
GTCCTACAGTGTGCATTTCTCATTT57500.18028101891696569No Hit
TATCAACGCAGAGTACTTTTTTTTT48500.15206312030387542No Hit
GGTATCAACGCAGAGTACTTTTTTT45380.14228091545133745No Hit
CTGTAGGACCTGGAATATGGCGAGA43480.1363238035219073No Hit
CTGAAGGACCTGGAATATGGCGAGA39990.12538152950416448No Hit
TTTCTAAATTTTCCACCTTTTTCAG32180.10089466415213837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAT500.001500991413.29901111
TAGGACC16900.012.6511564
TGTAGGA51350.011.121282
GGCGAGG20850.011.02210219
AGGACCT31400.010.7433555
GTAGGAC50950.010.7050443
AGGACGT45950.010.6503275
CTGTAGG52350.010.629761
GGACCTG30000.010.4807246
GGACGTG44700.010.4766866
GACGTGG22150.010.4642337
AGGACCG1002.3933837E-510.452835
TTAGGAC29000.010.354043
TGGCGAG47450.010.26763318
AAACGCC856.6205603E-410.05492319
TAGGACG50600.09.990694
CCACCTT12800.09.9439513
AGCACCG1151.0239242E-59.9157295
TAGGACA14500.09.8956274
GGTATCA23300.09.87971
Produced by FastQC (version 0.11.5)