##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512481_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3189465 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.520726203297418 33.0 31.0 34.0 30.0 34.0 2 31.8046440390473 33.0 31.0 34.0 30.0 34.0 3 31.974406679490134 34.0 31.0 34.0 30.0 34.0 4 35.45435268924412 37.0 35.0 37.0 33.0 37.0 5 34.933296336532926 37.0 35.0 37.0 32.0 37.0 6 35.164243846538525 37.0 35.0 37.0 32.0 37.0 7 35.11743662338355 37.0 35.0 37.0 32.0 37.0 8 35.09111998407256 37.0 35.0 37.0 32.0 37.0 9 36.678160757368396 39.0 37.0 39.0 33.0 39.0 10 36.61761988295843 39.0 37.0 39.0 32.0 39.0 11 36.66252678740792 39.0 37.0 39.0 33.0 39.0 12 36.589804559698884 39.0 37.0 39.0 32.0 39.0 13 36.56803601857992 39.0 37.0 39.0 32.0 39.0 14 37.839752434969505 40.0 38.0 41.0 32.0 41.0 15 37.84422528543188 40.0 38.0 41.0 32.0 41.0 16 37.82825301422025 40.0 38.0 41.0 32.0 41.0 17 37.79637462709263 40.0 38.0 41.0 32.0 41.0 18 37.735186308675594 40.0 38.0 41.0 32.0 41.0 19 37.76425983668107 40.0 38.0 41.0 32.0 41.0 20 37.745493679974544 40.0 38.0 41.0 32.0 41.0 21 37.687031210563525 40.0 38.0 41.0 32.0 41.0 22 37.62451727797609 40.0 38.0 41.0 32.0 41.0 23 37.520057752632496 40.0 38.0 41.0 32.0 41.0 24 37.495859650442945 40.0 38.0 41.0 32.0 41.0 25 37.43707894584202 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44018908804152446 1101 2 0.5362698804886286 1101 3 0.5569799827899402 1101 4 0.5494005292839361 1101 5 0.2716354447191449 1101 6 0.47853502458804087 1101 7 0.6316403707427583 1101 8 0.6438376432919952 1101 9 0.8302494577937267 1101 10 0.8532025657175595 1101 11 0.8470588523695213 1101 12 0.8057601219619102 1101 13 0.8405125726351272 1101 14 1.0083510092649064 1101 15 1.0859767001229983 1101 16 0.9897583318142011 1101 17 0.9804637329332806 1101 18 0.9472657980529107 1101 19 0.9960263876475253 1101 20 0.9289919914201406 1101 21 0.9768459250337429 1101 22 1.0567526271885725 1101 23 1.058307036814277 1101 24 1.020204836894301 1101 25 1.052721937847437 1102 1 -0.07933922898320134 1102 2 0.07678997458604542 1102 3 0.14533217294664524 1102 4 0.1185404068483038 1102 5 -0.24217943647833096 1102 6 -0.03904731065065192 1102 7 -9.002140660214764E-4 1102 8 0.1312735341293063 1102 9 0.10176122299827028 1102 10 0.09244100638895958 1102 11 0.09503453176013466 1102 12 0.09747270703711308 1102 13 0.25889714283881915 1102 14 0.28088080072468813 1102 15 0.2912917811412328 1102 16 0.24537110036119714 1102 17 0.3639599000076643 1102 18 0.35696005714172685 1102 19 0.31718263840441807 1102 20 0.31605654866576316 1102 21 0.35299073151036 1102 22 0.3725292336264303 1102 23 0.3358032069162462 1102 24 0.2907655612143074 1102 25 0.24094023295243971 1103 1 -0.13041349689002857 1103 2 -0.043000482066407386 1103 3 0.0268894992715758 1103 4 0.11664474671986369 1103 5 -0.08683366281598381 1103 6 0.07141272638269669 1103 7 0.2201207233285345 1103 8 0.2285952368059938 1103 9 0.34105691866655263 1103 10 0.2578966216846439 1103 11 0.2722522631010236 1103 12 0.30381020835084627 1103 13 0.37156761655592874 1103 14 0.4161994562398661 1103 15 0.34740549896868345 1103 16 0.273930979470407 1103 17 0.34132692981687995 1103 18 0.3631405504679819 1103 19 0.4113565755279325 1103 20 0.4203066198837391 1103 21 0.3501018225698047 1103 22 0.3612977088149165 1103 23 0.3298313546126579 1103 24 0.27519708616410554 1103 25 0.35491959633284154 1104 1 -0.23165198246583074 1104 2 -0.14915948318154548 1104 3 -0.17653290816779688 1104 4 -0.08978649757771962 1104 5 -0.4453843386119942 1104 6 -0.2836222312995673 1104 7 -0.1779495660321686 1104 8 -0.13901885257498492 1104 9 -0.085314469119524 1104 10 -0.0020804624877825972 1104 11 -0.030585398587248847 1104 12 -0.10050824870261721 1104 13 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24 0.4687421194758059 1105 25 0.42184067218106236 1106 1 0.1076223168778192 1106 2 0.22015929145932844 1106 3 0.19124594234679648 1106 4 0.2078273373419961 1106 5 -0.0887062273583652 1106 6 0.1653569896801983 1106 7 0.2570277441931168 1106 8 0.25504434908995677 1106 9 0.36534466118460784 1106 10 0.3468058639667433 1106 11 0.2965644436555195 1106 12 0.3325525685187998 1106 13 0.3243845759248174 1106 14 0.4172172131789793 1106 15 0.457485848614148 1106 16 0.44378877366356306 1106 17 0.4932533147705911 1106 18 0.5248876222020868 1106 19 0.5136148333804655 1106 20 0.45871020864430534 1106 21 0.3098886844894011 1106 22 0.3621917918338937 1106 23 0.40224230393523897 1106 24 0.4351449074651512 1106 25 0.5159302976617255 1107 1 0.09486905983795779 1107 2 0.05916274504986063 1107 3 0.10331047260783066 1107 4 0.1489778142237128 1107 5 -0.08335542189928447 1107 6 0.11755330453375734 1107 7 0.23518097679170324 1107 8 0.24148100994872124 1107 9 0.2940883390284128 1107 10 0.3615278915318427 1107 11 0.3247747017704512 1107 12 0.232775638556447 1107 13 0.250502179783922 1107 14 0.25671494575058773 1107 15 0.3495943814376332 1107 16 0.25406480228150485 1107 17 0.3479582648399031 1107 18 0.3060555599721795 1107 19 0.3830190057660019 1107 20 0.3142008656395632 1107 21 0.27955472888675814 1107 22 0.3421223465459562 1107 23 0.35803515526641405 1107 24 0.3019118511247072 1107 25 0.33728306701629407 1108 1 -0.094850475418923 1108 2 0.041457858990362695 1108 3 0.07646602366336452 1108 4 0.07717797351478595 1108 5 -0.2110612872005575 1108 6 -0.0755617738992882 1108 7 0.08833454502460825 1108 8 0.12551906878484687 1108 9 0.18242232424307758 1108 10 0.03212783248526563 1108 11 0.13429976301767965 1108 12 0.04057489772393552 1108 13 0.18802298461519484 1108 14 0.21299646534885142 1108 15 0.26045611988142525 1108 16 0.3269433504204855 1108 17 0.436625159807015 1108 18 0.37582755078531704 1108 19 0.3045453930055757 1108 20 0.2340139448535652 1108 21 0.31299368565595387 1108 22 0.2702197949761427 1108 23 0.39820832610240586 1108 24 0.4782604767746861 1108 25 0.5704641432622353 1109 1 -0.139700677021537 1109 2 -0.06635529452789157 1109 3 -0.0671198282940999 1109 4 -0.05891795857333193 1109 5 -0.3524509035557273 1109 6 -0.1773846817626179 1109 7 -0.06018937784474332 1109 8 -0.026902432798351583 1109 9 -0.08413035061230545 1109 10 -0.03786693439742095 1109 11 -0.08637738048904708 1109 12 -0.16656325325895693 1109 13 0.003398009223111842 1109 14 -0.13395415841620206 1109 15 -0.18510860605766766 1109 16 -0.3723963289375618 1109 17 -0.4526701325084872 1109 18 -0.7141536477741965 1109 19 -0.5679779903124782 1109 20 -0.5085467596386906 1109 21 -0.4985600696277075 1109 22 -0.5229872276750669 1109 23 -0.48742030418630833 1109 24 -0.4091412489940538 1109 25 -0.5447058651094281 1110 1 -0.09330159937245952 1110 2 -0.007459664203690153 1110 3 -0.020582857649987574 1110 4 0.046593164794934694 1110 5 -0.222998108924358 1110 6 -0.018811567832358378 1110 7 0.11591146789896101 1110 8 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2314 14 -2.4000919731912305 2314 15 -1.7587227008183817 2314 16 -1.4036948132435683 2314 17 -1.3607121870496002 2314 18 -1.1883581321995464 2314 19 -1.0579156320358294 2314 20 -1.0228626833766086 2314 21 -1.022552341975242 2314 22 -1.0555574926231515 2314 23 -1.0233080145336046 2314 24 -1.0379025122712946 2314 25 -0.9135408991230349 2315 1 -0.8121976478973671 2315 2 -0.7254721622774589 2315 3 -0.583060648499302 2315 4 -0.42647525721400825 2315 5 -0.223807053879014 2315 6 -0.4293960896786899 2315 7 -0.4705430776601247 2315 8 -0.7786431299281915 2315 9 -0.7367732433578666 2315 10 -0.6847544912242469 2315 11 -0.6260720771916581 2315 12 -0.6612438333456367 2315 13 -0.6295181646500652 2315 14 -0.8084025481031105 2315 15 -0.9722686364386419 2315 16 -0.8685813402580678 2315 17 -0.9120375361800228 2315 18 -0.8270170341295042 2315 19 -0.7917308392522386 2315 20 -0.7539967777873855 2315 21 -0.6771932963845018 2315 22 -0.6944142360582006 2315 23 -0.7275003928978236 2315 24 -0.6842103992820299 2315 25 -0.7329738197255367 2316 1 -0.7078256008687198 2316 2 -0.7181434813076031 2316 3 -0.8329396360962633 2316 4 -0.7238311324960804 2316 5 -0.6196701429611906 2316 6 -0.892443792171143 2316 7 -1.4693507395282808 2316 8 -1.2088955952645435 2316 9 -1.274476586803921 2316 10 -1.3626491966859078 2316 11 -1.3942452124322458 2316 12 -1.330078182375516 2316 13 -1.2652477603198804 2316 14 -1.4264104407663112 2316 15 -1.4060022089047166 2316 16 -1.579999584242941 2316 17 -1.5819683160759368 2316 18 -1.650556157138027 2316 19 -1.6070695050295498 2316 20 -1.7341206947256396 2316 21 -1.925796747038845 2316 22 -1.8409088136157976 2316 23 -1.804971016027224 2316 24 -1.8385104030292467 2316 25 -1.8003823157315466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27372.0 3 2.0 4 628.0 5 4325.0 6 5380.0 7 4232.0 8 3623.0 9 3210.0 10 2934.0 11 2780.0 12 2612.0 13 2693.0 14 2637.0 15 2691.0 16 2795.0 17 2977.0 18 3215.0 19 3828.0 20 4641.0 21 5977.0 22 7998.0 23 10108.0 24 12306.0 25 14774.0 26 17336.0 27 19969.0 28 23377.0 29 28830.0 30 37028.0 31 48236.0 32 63477.0 33 84060.0 34 113500.0 35 156839.0 36 233477.0 37 433487.0 38 1796111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.321528982022244 20.078154332203628 13.493896704182587 25.106419981591543 2 14.304800175083136 20.88966940516257 42.10009462766928 22.705435792085012 3 18.357301013283234 25.385664039450262 30.4704533782483 25.786581569018203 4 12.155537502433878 17.662297023879137 39.18503219932068 30.997133274366305 5 12.383803045515238 38.636716496838844 36.684002309746255 12.295478147899665 6 30.991350652934756 38.40495126510029 18.285999222248705 12.317698859716247 7 27.37756933028857 31.802527920583678 23.083378229125966 17.73652452000178 8 24.07359442290892 38.849423934305115 20.834381191114833 16.242600451671127 9 26.212986077945565 14.989923075451658 20.849779348748765 37.94731149785401 10 14.28062498255592 29.59977245080456 35.63681944178319 20.482783124856333 11 33.55425349985401 22.25564999170491 26.028050264799994 18.162046243641083 12 23.15269560984911 24.912720097146327 32.930522312042434 19.00406198096213 13 29.078488831574166 22.459380699936524 26.074379232222473 22.387751236266837 14 19.831812930925274 23.23910935734202 28.30598155950265 28.623096152230055 15 21.97506187806392 33.00627513967838 23.406700611144856 21.611962371112845 16 20.76679570458939 28.639049728989 29.606536248994587 20.987618317427017 17 20.813737854670585 29.679475561336382 28.855948067879012 20.65083851611402 18 20.628479296831642 27.836130567266874 31.607520580254366 19.927869555647113 19 23.12025165838246 27.18414700180567 28.240112648041027 21.455488691770842 20 23.131164471823634 27.994830450178494 29.067994880615633 19.80601019738224 21 21.922731553884493 27.188557783594174 27.86657394436411 23.022136718157228 22 22.54463249910641 29.27572130006459 27.882880058193127 20.29676614263587 23 21.130356098102403 28.940064626535005 28.7690294293413 21.160549846021294 24 22.58217643469749 27.371420114892437 29.570285236993083 20.476118213416996 25 21.85169671142236 28.221669847974457 28.912757754908036 21.01387568569515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 226.0 1 226.0 2 477.5 3 729.0 4 729.0 5 729.0 6 1902.0 7 3075.0 8 3075.0 9 3075.0 10 4690.0 11 6305.0 12 6305.0 13 6305.0 14 10970.0 15 15635.0 16 15635.0 17 15635.0 18 28580.5 19 41526.0 20 41526.0 21 41526.0 22 67416.5 23 93307.0 24 93307.0 25 93307.0 26 135185.0 27 177063.0 28 177063.0 29 177063.0 30 229837.0 31 282611.0 32 282611.0 33 282611.0 34 315884.0 35 349157.0 36 349157.0 37 349157.0 38 368415.5 39 387674.0 40 387674.0 41 387674.0 42 399439.0 43 411204.0 44 411204.0 45 411204.0 46 414906.5 47 418609.0 48 418609.0 49 418609.0 50 391552.0 51 364495.0 52 364495.0 53 364495.0 54 312286.5 55 260078.0 56 260078.0 57 260078.0 58 218264.5 59 176451.0 60 176451.0 61 176451.0 62 141385.5 63 106320.0 64 106320.0 65 106320.0 66 81184.0 67 56048.0 68 56048.0 69 56048.0 70 40347.5 71 24647.0 72 24647.0 73 24647.0 74 16989.5 75 9332.0 76 9332.0 77 9332.0 78 6371.5 79 3411.0 80 3411.0 81 3411.0 82 2268.0 83 1125.0 84 1125.0 85 1125.0 86 734.0 87 343.0 88 343.0 89 343.0 90 210.5 91 78.0 92 78.0 93 78.0 94 45.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.124566345766453 2 0.0038564461437890054 3 9.719498411175542E-4 4 0.00351156071629568 5 0.038752580761977316 6 0.024612278234750967 7 0.018780579188045644 8 0.013136999465427587 9 0.015551197457880868 10 0.022072667359572844 11 0.026085879606767906 12 0.027371361654697578 13 0.02611723282744912 14 0.012447228610440935 15 0.01667991340240448 16 0.015049545926981484 17 0.01188287063817913 18 0.007618832625534377 19 0.0019438996822351085 20 5.957111929430171E-4 21 4.0759186885574853E-4 22 0.002665023757902971 23 0.00197525290291632 24 0.001473601372016937 25 3.1353220681211426E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3189465.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.52397711597666 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05867439309559 52.45762748868622 2 13.274590119633157 17.396078784456257 3 3.5591642420153655 6.996317890374518 4 1.3167354077980182 3.4511096291341414 5 0.5870326310119933 1.923235634038071 6 0.3211546912723302 1.262599958497003 7 0.19165981482848912 0.8790819324611973 8 0.12101210248338579 0.6343355387102074 9 0.08985254338697707 0.5298746397030527 >10 0.42656253019267076 4.932830898609274 >50 0.028244079153316927 1.2574177874498822 >100 0.019865527077967734 2.669359429642714 >500 0.003108549768038225 1.408870321319893 >1k 0.002056425227734816 2.775656278743094 >5k 2.8694305503276503E-4 1.4256037881745636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 8792 0.27565751622921086 No Hit GTCCTACAGTGGACATTTCTAAATT 8458 0.26518554052168625 No Hit GTCCTAAAGTGTGTATTTCTCATTT 8033 0.2518604217321714 No Hit CTTTAGGACGTGAAATATGGCGAGG 7480 0.23452209069546145 No Hit GTATCAACGCAGAGTACTTTTTTTT 6981 0.21887683357553694 No Hit GTCCTACAGTGTGCATTTCTCATTT 5750 0.18028101891696569 No Hit TATCAACGCAGAGTACTTTTTTTTT 4850 0.15206312030387542 No Hit GGTATCAACGCAGAGTACTTTTTTT 4538 0.14228091545133745 No Hit CTGTAGGACCTGGAATATGGCGAGA 4348 0.1363238035219073 No Hit CTGAAGGACCTGGAATATGGCGAGA 3999 0.12538152950416448 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3218 0.10089466415213837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.1353220681211426E-5 0.0 6 0.0 0.0 0.0 3.1353220681211426E-5 0.0 7 0.0 0.0 0.0 3.1353220681211426E-5 0.0 8 0.0 0.0 0.0 3.1353220681211426E-5 0.0 9 0.0 0.0 0.0 3.1353220681211426E-5 0.0 10 0.0 0.0 0.0 3.1353220681211426E-5 0.0 11 0.0 0.0 0.0 3.1353220681211426E-5 0.0 12 0.0 0.0 0.0 3.1353220681211426E-5 0.0 13 0.0 0.0 0.0 3.1353220681211426E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGAT 50 0.0015009914 13.299011 11 TAGGACC 1690 0.0 12.651156 4 TGTAGGA 5135 0.0 11.12128 2 GGCGAGG 2085 0.0 11.022102 19 AGGACCT 3140 0.0 10.743355 5 GTAGGAC 5095 0.0 10.705044 3 AGGACGT 4595 0.0 10.650327 5 CTGTAGG 5235 0.0 10.62976 1 GGACCTG 3000 0.0 10.480724 6 GGACGTG 4470 0.0 10.476686 6 GACGTGG 2215 0.0 10.464233 7 AGGACCG 100 2.3933837E-5 10.45283 5 TTAGGAC 2900 0.0 10.35404 3 TGGCGAG 4745 0.0 10.267633 18 AAACGCC 85 6.6205603E-4 10.054923 19 TAGGACG 5060 0.0 9.99069 4 CCACCTT 1280 0.0 9.94395 13 AGCACCG 115 1.0239242E-5 9.915729 5 TAGGACA 1450 0.0 9.895627 4 GGTATCA 2330 0.0 9.8797 1 >>END_MODULE