FastQCFastQC Report
Thu 26 May 2016
SRR1512480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902425
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33880.37543286145663074No Hit
GTCCTACAGTGGACATTTCTAAATT22640.2508795744798737No Hit
CTGTAGGACGTGGAATATGGCAAGA21760.24112807158489627No Hit
GTCCTAAAGTGTGTATTTCTCATTT21600.23935507105853673No Hit
GGTATCAACGCAGAGTACTTTTTTT21210.23503338227553536No Hit
TATCAACGCAGAGTACTTTTTTTTT19040.21098706263678424No Hit
CTTTAGGACGTGAAATATGGCGAGG18610.206222123722193No Hit
GTACATGGGAAGCAGTGGTATCAAC14100.15624567138543372No Hit
GTCCTACAGTGTGCATTTCTCATTT11430.12665872510180903No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10970.12156134858852537No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10240.11347203368701No Hit
CCCATGTACTCTGCGTTGATACCAC10090.11180984569354793No Hit
CTGAAGGACCTGGAATATGGCGAGA9430.10449621852231486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGG350.001640695116.980211
TAGGACC3100.015.5904164
TGCGCCA456.857317E-414.74947812
GTGTAGG1900.013.0330551
GCGCCAC604.138132E-412.64875313
ATCCTAA801.8660767E-512.3814031
GGCGAGG5950.012.26032719
CTAGGGG550.003124053812.0610343
TGGACTG1052.803281E-711.7328445
GTCTTAC958.542731E-611.4690891
TGTCCAC3600.011.322968510
AGGACCT7050.011.2911825
CTAATAC855.452481E-511.1488553
GCAATAC600.00598150211.0559493
TCGAACT600.00599390911.05287119
TGTAGGA14000.010.9656952
CGAAATC1053.5194425E-610.84178813
TTACACC700.001521412210.8303174
CTTACTC700.001521412210.8303173
GGACGTG13850.010.8107676