FastQCFastQC Report
Thu 26 May 2016
SRR1512480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902425
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41370.45843144859683627No Hit
GGTATCAACGCAGAGTACTTTTTTT28020.3104967171787129No Hit
TATCAACGCAGAGTACTTTTTTTTT27640.306285840928609No Hit
CTGTAGGACGTGGAATATGGCAAGA27260.30207496467850514No Hit
GTCCTACAGTGGACATTTCTAAATT25150.27869352023713884No Hit
GTCCTAAAGTGTGTATTTCTCATTT24390.270271767736931No Hit
CTTTAGGACGTGAAATATGGCGAGG22800.2526525750062332No Hit
GTACATGGGAAGCAGTGGTATCAAC15760.17464055184641383No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13370.1481563564839183No Hit
GTCCTACAGTGTGCATTTCTCATTT12890.14283735490483973No Hit
CCCATGTACTCTGCGTTGATACCAC12330.13663185306258138No Hit
CTGAAGGACCTGGAATATGGCGAGA12260.13585616533229908No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11880.1316452890821952No Hit
CTTATACACATCTCCGAGCCCACGA9730.107820594509239No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9440.10460703105521234No Hit
GTCCTTCAGTGTGCATTTCTCATTT9110.1009502174695958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT755.826587E-815.2019964
TAGGACC3700.014.6370564
GTCGAGG701.0925371E-412.21115219
ATAGGAC1353.6925485E-1011.9645333
GGCGAGG5650.011.43069419
CTTAGAC752.0694523E-411.4014973
GGACAGC1251.8279025E-811.39966
TAGACTG855.309784E-511.17793755
TATACTG1451.2114469E-911.1393945
GTCTAAG600.005823245711.095861
TTAGACA951.3563562E-511.0014454
CTTAGGC951.3563562E-511.0014453
TAACACT1053.4549103E-610.8585684
TTTATAC700.001488719810.8585683
GGACCTG7500.010.766296
AGGACCT8050.010.740545
GCCTTAG803.7282443E-410.6995791
GGTGGAC803.7628028E-410.6889043
TTAGGAC8550.010.6680673
TGTAGGA14050.010.6170522
Produced by FastQC (version 0.11.5)