FastQCFastQC Report
Thu 26 May 2016
SRR1512479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42090.2512429332328919No Hit
GTACATGGGAAGCAGTGGTATCAAC36300.21668136080669936No Hit
CCCATGTACTCTGCGTTGATACCAC27070.1615857971635634No Hit
GGTATCAACGCAGAGTACTTTTTTT25790.1539452422921426No Hit
TATCAACGCAGAGTACTTTTTTTTT24010.14332009567407303No Hit
GTCCTACAGTGGACATTTCTAAATT19830.11836890867208948No Hit
GTCCTAAAGTGTGTATTTCTCATTT18770.11204157416919412No Hit
CTGTAGGACGTGGAATATGGCAAGA18280.10911667425747833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG350.002204541316.2475765
CGAGCCA456.873012E-414.74626810
GTATTAG1051.1008524E-813.2159191
TAGGACC3150.012.6370044
ATAATAC2000.011.3733033
GTACTAG700.001046102111.327931
GTTCTAG1501.0568328E-911.2335311
ACCGTCC600.006096516311.028598
GGCGAGG5000.010.98496519
CACACCG803.8535582E-410.6624725
GTATAGG2051.8189894E-1210.6372021
CAAGTCG1002.4615196E-510.4255286
GTCCTAA5900.010.4159361
GTCTTAT1353.5998164E-710.2790471
GTAGGAC11200.010.2393583
TTATACT2053.6379788E-1210.1712474
GTATAAT2153.6379788E-1210.1424481
GGACGTG12250.010.1353926
GTATAGA3050.010.07442951
AGGACGT12600.010.0042945
Produced by FastQC (version 0.11.5)