FastQCFastQC Report
Thu 26 May 2016
SRR1512479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53900.32173899028873537No Hit
GTACATGGGAAGCAGTGGTATCAAC40620.24246823349774455No Hit
GGTATCAACGCAGAGTACTTTTTTT35540.21214478135179327No Hit
TATCAACGCAGAGTACTTTTTTTTT35240.21035402630380398No Hit
CCCATGTACTCTGCGTTGATACCAC32370.19322246967804016No Hit
GTCCTACAGTGGACATTTCTAAATT21580.1288149797853601No Hit
CTGTAGGACGTGGAATATGGCAAGA21190.12648699822297407No Hit
GTCCTAAAGTGTGTATTTCTCATTT19840.11842860050702245No Hit
CTTTAGGACGTGAAATATGGCGAGG19030.11359356187745147No Hit
GAGTACATGGGAAGCAGTGGTATCA18210.10869883141294752No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16800.10028228268739803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG951.0441527E-611.99596618
CCAACGA907.4918844E-611.60686119
CGTGCGG600.005880245511.08225310
GTCCTAA6450.011.0619491
TTAGGAC7950.010.8777023
ATTAGAC700.001487522210.8606243
CGTTTCC803.780653E-410.68454514
GGCGAGG6250.010.63610619
CCGTTTC1002.4044248E-510.44773413
ATTTAGA3750.010.4011821
TGGCGAG11650.010.18970718
CTTAGAC1409.640644E-810.1818353
TATGGCG12100.010.04680816
AGGACGT13150.09.9730775
ATGGCGA12200.09.88631417
TCTAGAC1252.558936E-69.8831683
TCCTACA11250.09.8831682
TTTAGGA8950.09.8746742
TACTATA1356.4128835E-79.8550122
GTCTAGG1451.5839942E-79.841321
Produced by FastQC (version 0.11.5)