##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675271 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63199864380151 33.0 31.0 34.0 30.0 34.0 2 31.91069982110357 34.0 31.0 34.0 30.0 34.0 3 32.076736838398084 34.0 31.0 34.0 30.0 34.0 4 35.548519612647745 37.0 35.0 37.0 33.0 37.0 5 35.01649822625713 37.0 35.0 37.0 32.0 37.0 6 35.256337034426075 37.0 35.0 37.0 33.0 37.0 7 35.21732603262397 37.0 35.0 37.0 33.0 37.0 8 35.200253570914796 37.0 35.0 37.0 33.0 37.0 9 36.80959677568585 39.0 37.0 39.0 33.0 39.0 10 36.76490251427978 39.0 37.0 39.0 33.0 39.0 11 36.804054985730666 39.0 37.0 39.0 33.0 39.0 12 36.73065790549708 39.0 37.0 39.0 33.0 39.0 13 36.69013789410788 39.0 37.0 39.0 33.0 39.0 14 38.000760473977046 40.0 38.0 41.0 33.0 41.0 15 37.98456130381294 40.0 38.0 41.0 33.0 41.0 16 37.958246158382735 40.0 38.0 41.0 33.0 41.0 17 37.93655295173139 40.0 38.0 41.0 33.0 41.0 18 37.88489444394369 40.0 38.0 41.0 33.0 41.0 19 37.91236044795141 40.0 38.0 41.0 33.0 41.0 20 37.89506115726948 40.0 38.0 41.0 33.0 41.0 21 37.839084542142736 40.0 38.0 41.0 33.0 41.0 22 37.774653772434434 40.0 38.0 41.0 33.0 41.0 23 37.66620624364655 40.0 38.0 41.0 32.0 41.0 24 37.6323239643019 40.0 38.0 41.0 32.0 41.0 25 37.57197432534796 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13140672601939585 1101 2 0.18919383749463847 1101 3 0.14757997626476538 1101 4 0.17374676719811077 1101 5 -0.10462793629621814 1101 6 0.1827791151811553 1101 7 0.28739989652388687 1101 8 0.36947169080985987 1101 9 0.434912067868062 1101 10 0.5202361531923998 1101 11 0.4590847277949166 1101 12 0.3803121721940528 1101 13 0.5017221896644628 1101 14 0.607541047616607 1101 15 0.5133273887722041 1101 16 0.5111447044283821 1101 17 0.6330103246200451 1101 18 0.5215501741560686 1101 19 0.4801255804148923 1101 20 0.42853632907927874 1101 21 0.4933300730107888 1101 22 0.5059410294129165 1101 23 0.531313145709646 1101 24 0.4902280481525807 1101 25 0.4785429284832148 1102 1 0.17623054825059725 1102 2 0.3386187378097638 1102 3 0.35879406936384584 1102 4 0.30292602600840723 1102 5 -0.08691054468686588 1102 6 0.2051068448351998 1102 7 0.299491160452412 1102 8 0.4073252880240119 1102 9 0.5158834096924991 1102 10 0.5340980338623709 1102 11 0.4408033203931154 1102 12 0.4490294992642916 1102 13 0.6009300421111945 1102 14 0.6286453400293368 1102 15 0.678743960734721 1102 16 0.55800291198058 1102 17 0.5673684269454213 1102 18 0.7206561670343135 1102 19 0.6529669572868784 1102 20 0.6529051785712383 1102 21 0.5661105586255815 1102 22 0.6550011433946139 1102 23 0.629112361699633 1102 24 0.7664358416722905 1102 25 0.7534571335436411 1103 1 -0.12624826214902996 1103 2 -0.04125536144803377 1103 3 0.04321883121247083 1103 4 0.003363883391742206 1103 5 -0.16397898100107966 1103 6 0.012205796317239503 1103 7 0.1077394540767358 1103 8 0.2045713320812368 1103 9 0.2500870995540865 1103 10 0.29918661000874636 1103 11 0.19871900136717358 1103 12 0.29256625811945014 1103 13 0.39155687563340535 1103 14 0.4695485246983395 1103 15 0.5199096990865826 1103 16 0.5167748394550387 1103 17 0.432288129277417 1103 18 0.43597831772405726 1103 19 0.5055591390811927 1103 20 0.3899764190302264 1103 21 0.4415654507419191 1103 22 0.4658312149003052 1103 23 0.35530012687662094 1103 24 0.48836470034071766 1103 25 0.4751752348339977 1104 1 -0.03874416198397057 1104 2 -0.03609923944115323 1104 3 0.037327749137254784 1104 4 0.04104505806491687 1104 5 -0.32399241726390215 1104 6 -0.16403640021353993 1104 7 0.014511786601438814 1104 8 -0.002543572523052262 1104 9 0.010236652526465662 1104 10 0.10604647119075139 1104 11 0.11798653209429233 1104 12 0.043184133260432134 1104 13 0.008541820353535456 1104 14 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11 -0.7647340158680151 2314 12 -0.6830648642552788 2314 13 -1.0007843342557905 2314 14 -2.652654208577175 2314 15 -1.7464536268630937 2314 16 -1.3089849690822462 2314 17 -1.0870986810876246 2314 18 -0.9795013482295829 2314 19 -0.9227058201398037 2314 20 -0.8589752173234828 2314 21 -0.8636370846415033 2314 22 -0.8171848858597031 2314 23 -0.7213930539531219 2314 24 -0.8713856797500412 2314 25 -0.9644382590987632 2315 1 -0.7139982832930336 2315 2 -0.6563025580744153 2315 3 -0.6154305112893255 2315 4 -0.4607962286340026 2315 5 -0.44428261958957904 2315 6 -0.5042319262969954 2315 7 -0.6271037751852759 2315 8 -0.9560192440098731 2315 9 -0.853965215526884 2315 10 -0.807061201988418 2315 11 -0.8186790336858323 2315 12 -0.812599730333254 2315 13 -0.814883534142723 2315 14 -0.8584206553746938 2315 15 -1.069950440024897 2315 16 -0.9789398867274457 2315 17 -0.9626012849105834 2315 18 -1.0127485456514265 2315 19 -0.8825850705131515 2315 20 -0.8488397416256532 2315 21 -0.8026756187271076 2315 22 -0.8361827647650486 2315 23 -0.7985329329228961 2315 24 -0.9578098945241251 2315 25 -0.9234419473716358 2316 1 -0.6029049465835143 2316 2 -0.6433313320741334 2316 3 -0.606907638200834 2316 4 -0.638543001011783 2316 5 -0.39533876122513334 2316 6 -0.7514773693016181 2316 7 -1.0657618439841912 2316 8 -0.9719306601943529 2316 9 -1.0249641787184771 2316 10 -1.115820522143217 2316 11 -1.222560392691868 2316 12 -1.0447259061526921 2316 13 -1.1115724197618846 2316 14 -1.3030908442243856 2316 15 -1.2956889521535047 2316 16 -1.3374240933637012 2316 17 -1.396609969464194 2316 18 -1.4703138252304058 2316 19 -1.4525910244624356 2316 20 -1.5455424855750408 2316 21 -1.5570906089920555 2316 22 -1.5055572236913548 2316 23 -1.4552133324534253 2316 24 -1.6322905493216737 2316 25 -1.4741343506114077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13293.0 3 0.0 4 339.0 5 2339.0 6 2697.0 7 2254.0 8 1875.0 9 1636.0 10 1443.0 11 1451.0 12 1375.0 13 1325.0 14 1390.0 15 1346.0 16 1433.0 17 1475.0 18 1559.0 19 1919.0 20 2331.0 21 3028.0 22 3895.0 23 5052.0 24 6197.0 25 7250.0 26 8564.0 27 9575.0 28 11364.0 29 14074.0 30 18208.0 31 23818.0 32 30992.0 33 41491.0 34 56791.0 35 79001.0 36 118205.0 37 219072.0 38 977214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.838204847447415 18.84978316064282 13.1861399725544 25.12587201935536 2 15.352352799314229 20.264076561787398 39.74394638000568 24.639624258892688 3 18.702499683629686 24.41041705964237 29.986891524379615 26.90019173234833 4 12.907202192916479 16.863179108077066 37.15368562307338 33.07593307593308 5 13.503313350320587 37.62212698160564 35.460223374213015 13.414336293860762 6 32.74585678572431 36.39323472532118 17.53127300583277 13.329635483121741 7 28.33013424241248 31.51475711287766 21.8019588585962 18.353149786113658 8 25.79829976239657 36.001635762301056 20.279333277613937 17.92073119768844 9 26.72776835169926 14.765059802199612 19.82511274478783 38.6820591013133 10 15.327611241665235 28.604189354425785 34.22257408150398 21.845625322405 11 34.38291312127985 22.180891302933013 24.19345497361223 19.24274060217491 12 23.329728993875808 24.63594877817669 31.238696634231594 20.79562559371591 13 29.06975563942321 21.532294321737744 25.545284056408263 23.852665982430782 14 21.287631128378653 21.714955022936273 27.366340392913514 29.631073455771563 15 23.67404884944932 29.74649915463898 23.37339644088682 23.206055555024882 16 22.6071963955337 27.77831839730919 27.20083723723652 22.413647969920593 17 22.25250944137393 28.15965145420441 27.341598042832732 22.24624106158892 18 22.150320929166348 26.9864058798808 29.023613605104302 21.839659585848555 19 23.62807389526013 26.758498504091367 27.750418448505165 21.863009152143338 20 23.827525279658083 26.78688681159939 27.84290199730191 21.54268591144061 21 23.247302957568998 26.287008412420516 27.510578948593682 22.955109681416804 22 23.543088617508605 27.237345259314882 27.04662426830593 22.17294185487058 23 22.652615719447457 27.029250189674652 27.5672039239821 22.750930166895788 24 23.16067202110736 26.696930896674814 27.927616557578144 22.21478052463968 25 23.001440365696492 26.655420711539463 27.58750982678006 22.755629095983988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 276.0 1 276.0 2 425.5 3 575.0 4 575.0 5 575.0 6 1448.0 7 2321.0 8 2321.0 9 2321.0 10 2951.5 11 3582.0 12 3582.0 13 3582.0 14 5453.5 15 7325.0 16 7325.0 17 7325.0 18 12962.5 19 18600.0 20 18600.0 21 18600.0 22 29082.0 23 39564.0 24 39564.0 25 39564.0 26 56308.5 27 73053.0 28 73053.0 29 73053.0 30 93344.0 31 113635.0 32 113635.0 33 113635.0 34 129130.5 35 144626.0 36 144626.0 37 144626.0 38 158827.5 39 173029.0 40 173029.0 41 173029.0 42 185261.0 43 197493.0 44 197493.0 45 197493.0 46 211379.0 47 225265.0 48 225265.0 49 225265.0 50 219154.0 51 213043.0 52 213043.0 53 213043.0 54 192576.5 55 172110.0 56 172110.0 57 172110.0 58 149519.5 59 126929.0 60 126929.0 61 126929.0 62 105025.5 63 83122.0 64 83122.0 65 83122.0 66 64508.5 67 45895.0 68 45895.0 69 45895.0 70 33674.5 71 21454.0 72 21454.0 73 21454.0 74 15064.0 75 8674.0 76 8674.0 77 8674.0 78 5961.0 79 3248.0 80 3248.0 81 3248.0 82 2154.5 83 1061.0 84 1061.0 85 1061.0 86 688.0 87 315.0 88 315.0 89 315.0 90 190.5 91 66.0 92 66.0 93 66.0 94 37.5 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12791960226136548 2 0.004476887619973127 3 0.0011341448637265253 4 0.0035218182610455263 5 0.04070983142428897 6 0.025309338011581414 7 0.018862619838820108 8 0.01259497717085773 9 0.014863266898310782 10 0.021429368740938034 11 0.026383791040374964 12 0.026145023700643057 13 0.02447365232251976 14 0.011639907811930129 15 0.014743883228444832 16 0.014206656714048057 17 0.01175929148179608 18 0.008058397715951628 19 0.002089214222654126 20 6.566101842627253E-4 21 5.969183493297502E-4 22 0.002924899911715776 23 0.002029522387721151 24 0.0015519877082573507 25 1.1938366986595005E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1675271.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.64542888323746 #Duplication Level Percentage of deduplicated Percentage of total 1 80.14142017024064 55.013421588979064 2 13.29077791381258 18.247023001710488 3 3.5837325375930478 7.380205711376629 4 1.3105429281474128 3.5985112549029195 5 0.5872295452253908 2.015531199245272 6 0.3034568433447764 1.2498555095373347 7 0.18226437517935828 0.8758131343025645 8 0.121720526102494 0.6684446174559201 9 0.0834625971336629 0.515639319835424 >10 0.3583649776853319 4.202562326273722 >50 0.019205305492588445 0.9052336312170352 >100 0.014605931581474768 2.150923221299531 >500 0.0017385667358312442 0.8752140584138208 >1k 0.0013908533886649952 1.9799875688435937 >5k 8.69283367915622E-5 0.32163385660670174 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5390 0.32173899028873537 No Hit GTACATGGGAAGCAGTGGTATCAAC 4062 0.24246823349774455 No Hit GGTATCAACGCAGAGTACTTTTTTT 3554 0.21214478135179327 No Hit TATCAACGCAGAGTACTTTTTTTTT 3524 0.21035402630380398 No Hit CCCATGTACTCTGCGTTGATACCAC 3237 0.19322246967804016 No Hit GTCCTACAGTGGACATTTCTAAATT 2158 0.1288149797853601 No Hit CTGTAGGACGTGGAATATGGCAAGA 2119 0.12648699822297407 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1984 0.11842860050702245 No Hit CTTTAGGACGTGAAATATGGCGAGG 1903 0.11359356187745147 No Hit GAGTACATGGGAAGCAGTGGTATCA 1821 0.10869883141294752 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1680 0.10028228268739803 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.1938366986595004E-4 0.0 0.0 0.0 0.0 7 1.1938366986595004E-4 0.0 0.0 0.0 0.0 8 1.1938366986595004E-4 0.0 0.0 0.0 0.0 9 1.1938366986595004E-4 0.0 0.0 5.969183493297502E-5 0.0 10 1.1938366986595004E-4 0.0 0.0 5.969183493297502E-5 0.0 11 1.1938366986595004E-4 0.0 0.0 5.969183493297502E-5 0.0 12 1.1938366986595004E-4 0.0 0.0 5.969183493297502E-5 0.0 13 1.1938366986595004E-4 0.0 0.0 5.969183493297502E-5 5.969183493297502E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 95 1.0441527E-6 11.995966 18 CCAACGA 90 7.4918844E-6 11.606861 19 CGTGCGG 60 0.0058802455 11.082253 10 GTCCTAA 645 0.0 11.061949 1 TTAGGAC 795 0.0 10.877702 3 ATTAGAC 70 0.0014875222 10.860624 3 CGTTTCC 80 3.780653E-4 10.684545 14 GGCGAGG 625 0.0 10.636106 19 CCGTTTC 100 2.4044248E-5 10.447734 13 ATTTAGA 375 0.0 10.401182 1 TGGCGAG 1165 0.0 10.189707 18 CTTAGAC 140 9.640644E-8 10.181835 3 TATGGCG 1210 0.0 10.046808 16 AGGACGT 1315 0.0 9.973077 5 ATGGCGA 1220 0.0 9.886314 17 TCTAGAC 125 2.558936E-6 9.883168 3 TCCTACA 1125 0.0 9.883168 2 TTTAGGA 895 0.0 9.874674 2 TACTATA 135 6.4128835E-7 9.855012 2 GTCTAGG 145 1.5839942E-7 9.84132 1 >>END_MODULE