FastQCFastQC Report
Thu 26 May 2016
SRR1512478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302564
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT83930.25413587745763594No Hit
CTGTAGGACGTGGAATATGGCAAGA77620.23502951040464318No Hit
GTCCTAAAGTGTGTATTTCTCATTT74530.22567314365444546No Hit
CTTTAGGACGTGAAATATGGCGAGG61550.1863703474028058No Hit
GTACATGGGAAGCAGTGGTATCAAC58820.1781040427982622No Hit
GTATCAACGCAGAGTACTTTTTTTT56980.1725326140538079No Hit
GTCCTACAGTGTGCATTTCTCATTT46070.13949767513967937No Hit
CCCATGTACTCTGCGTTGATACCAC42300.1280823021143572No Hit
CTGAAGGACCTGGAATATGGCGAGA35410.10721972382669949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13300.012.8273564
GGCGAGG19150.011.71959919
GACGTAA600.005980365411.0576567
AGGACGT47400.010.9376825
GGACGTG47050.010.7571676
GTCCTAA23950.010.6783661
GACGTGG24300.010.6481137
TGTAGGA49350.010.6400952
CACCTTT12300.010.56723714
CTAGGAC4850.010.55281453
AGGACCT28050.010.5423645
GTAGGAC48950.010.4558023
TATGTCG2001.8189894E-1210.43563516
GGACCTG27350.010.4309886
GTGTAGG6850.010.4191551
CCACCTT12450.010.35961513
TCCAACG2750.010.32988218
GAAATGT13500.010.1800646
AATGTCC13200.010.1707968
AATACGG1409.890391E-810.16504414
Produced by FastQC (version 0.11.5)