FastQCFastQC Report
Thu 26 May 2016
SRR1512478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302564
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA94210.2852632076168698No Hit
GTCCTACAGTGGACATTTCTAAATT90590.27430202715223684No Hit
GTCCTAAAGTGTGTATTTCTCATTT80670.24426475913865714No Hit
GTATCAACGCAGAGTACTTTTTTTT74310.2250069945654346No Hit
CTTTAGGACGTGAAATATGGCGAGG73860.223644416883367No Hit
GTACATGGGAAGCAGTGGTATCAAC69160.2094130499817717No Hit
CCCATGTACTCTGCGTTGATACCAC51740.15666615393373148No Hit
TATCAACGCAGAGTACTTTTTTTTT50280.15224534634302317No Hit
GTCCTACAGTGTGCATTTCTCATTT47950.14519022190031744No Hit
GGTATCAACGCAGAGTACTTTTTTT47460.14370652620206603No Hit
CTGAAGGACCTGGAATATGGCGAGA44250.13398680540331692No Hit
CTGTAGGACCTGGAATATGGCGAGA36410.11024767423129422No Hit
GTCCTTCAGTGTGCATTTCTCATTT33590.10170885409033709No Hit
ATTTAGAAATGTCCACTGTAGGACG33500.10143633855392356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14800.013.0980414
TCCAACG3750.012.91518218
CCAACGA2400.012.66155919
CACCTTT11250.011.22811314
GGCGAGG20050.011.03544819
ATAAGAC3300.010.9416093
GGACGTA1053.4711193E-610.856716
TGTAGGA48350.010.749852
AGGACGT47550.010.6917245
AGGACCT30950.010.59260755
CTGTAGG48250.010.4900821
GGACCTG29900.010.4845316
GTCCTAC42600.010.4743681
TTAGGAC28250.010.426893
GGACGTG47550.010.4086286
CCACCTT11450.010.36966613
GTAGGAC49100.010.1986033
ACCTTTT12250.010.15647115
TAGAACG750.00264314510.1364
GCGCCAC1606.195478E-910.09227813
Produced by FastQC (version 0.11.5)