FastQCFastQC Report
Thu 26 May 2016
SRR1512477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598853
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC62620.39165576822884907No Hit
GTATCAACGCAGAGTACTTTTTTTT49110.30715769367165086No Hit
CCCATGTACTCTGCGTTGATACCAC43150.26988097092102903No Hit
GGTATCAACGCAGAGTACTTTTTTT31050.19420171835684708No Hit
TATCAACGCAGAGTACTTTTTTTTT29320.18338146158527394No Hit
GTCCTAAAGTGTGTATTTCTCATTT27740.17349937736614937No Hit
GTCCTACAGTGGACATTTCTAAATT27650.17293647383468022No Hit
GAGTACATGGGAAGCAGTGGTATCA27330.17093503905612337No Hit
CTGTAGGACGTGGAATATGGCAAGA25400.1588638855479522No Hit
CTTTAGGACGTGAAATATGGCGAGG23240.14535420079269326No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16980.10620113293717433No Hit
GTCCTACAGTGTGCATTTCTCATTT16790.1050127810374062No Hit
GTATCAACGCAGAGTACATGGGAAG16110.10075973213297283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTC400.00538020614.20933519
TAGGACC5950.013.0616414
GTCTTAG1950.011.1870821
AGGACCT12050.011.0113825
TCCCGTT700.001521021110.83160511
ACGGGGA700.001553212810.804448
GGACCTG11950.010.7862846
CACCTTT5300.010.73954614
ATTTAGA6250.010.3124551
TCTAGTA1201.5706592E-610.2671372
AATGTCC5500.010.1414398
TTTAGAA7050.010.0822942
TGTAGGA19850.010.0741672
GGACGTG18150.010.0259496
AGGACGT18350.09.9683235
TGAGTTA3150.09.93115512
TTTCCAC4900.09.86418710
TTCCACC5700.09.81020411
AAATGTC5900.09.798937
GTAGGAC19250.09.7976793