FastQCFastQC Report
Thu 26 May 2016
SRR1512477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598853
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC71400.4465701349655034No Hit
GTATCAACGCAGAGTACTTTTTTTT59870.3744559381006259No Hit
CCCATGTACTCTGCGTTGATACCAC52740.32986146944090544No Hit
GGTATCAACGCAGAGTACTTTTTTT42130.26350139756437896No Hit
TATCAACGCAGAGTACTTTTTTTTT41250.25799745192334755No Hit
GAGTACATGGGAAGCAGTGGTATCA30900.19326354580439853No Hit
GTCCTAAAGTGTGTATTTCTCATTT30300.1895108555946044No Hit
CTGTAGGACGTGGAATATGGCAAGA29950.1873217863055578No Hit
CTTTAGGACGTGAAATATGGCGAGG29470.18431963413772248No Hit
GTCCTACAGTGGACATTTCTAAATT28900.18075457843841805No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19990.12502712882297495No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19490.12189988698147985No Hit
CATGTACTCTGCGTTGATACCACTG19280.12058644540805188No Hit
GTCCTACAGTGTGCATTTCTCATTT18980.11871010030315482No Hit
GTATCAACGCAGAGTACATGGGAAG18820.11770938291387639No Hit
GCGTTGATACCACTGCTTCCCATGT17740.11095454053624693No Hit
TATCAACGCAGAGTACATGGGAAGC17340.10845274706305082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG851.7249477E-814.52412518
GTACTAG907.325845E-611.6301671
CGGTTTC907.470264E-611.60979213
GGCGAGG7650.011.41960819
CCTACAC1751.8189894E-1211.4019163
CGAAATC1104.975227E-711.22599713
TAGGACC5400.011.0855434
TGGCGAA951.3656469E-510.99599818
GCCGGTT803.7736705E-410.6866211
ACGAAAT1252.2420681E-710.63912412
CCAACGA1002.410636E-510.44521819
GAACAGT2200.010.36278256
GGACCTG11450.010.1214376
AGGACCT11850.010.1035955
AGGACGT19000.010.1023285
CCTTATC856.5829867E-410.06051352
TGGCGAG18850.010.02552918
GGACGTG19150.09.9704596
GTAGGAC16700.09.9567623
GACGTGA10900.09.9349627
Produced by FastQC (version 0.11.5)