FastQCFastQC Report
Thu 26 May 2016
SRR1512476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121991
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67910.32002963254792316No Hit
CTGTAGGACGTGGAATATGGCAAGA63920.3012265367760749No Hit
GTCCTAAAGTGTGTATTTCTCATTT62250.2933565693728202No Hit
GTATCAACGCAGAGTACTTTTTTTT55360.2608870631402301No Hit
CTTTAGGACGTGAAATATGGCGAGG50410.23755991425034317No Hit
GTCCTACAGTGTGCATTTCTCATTT35720.16833247643368893No Hit
GGTATCAACGCAGAGTACTTTTTTT33630.15848323579129223No Hit
TATCAACGCAGAGTACTTTTTTTTT31650.14915237623533747No Hit
GTACATGGGAAGCAGTGGTATCAAC29680.1398686422326956No Hit
CTGAAGGACCTGGAATATGGCGAGA27370.12898263941741506No Hit
CTGTAGGACCTGGAATATGGCGAGA25730.121254048674099No Hit
GTCCTTCAGTGTGCATTTCTCATTT25190.11870926879520224No Hit
ATTTAGAAATGTCCACTGTAGGACG23890.11258294686452487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC602.6942187E-514.1783198
TAGGACC11150.013.7700164
GTGTAGG3250.012.20642951
ATACGGC1103.8888174E-812.07458515
TGGCGAA1353.947207E-1011.92137118
TTGGCGA658.1185903E-411.67624517
GTTCTAG1705.456968E-1211.6679121
TACGGCA752.1011397E-411.38433716
AGGACCT21950.011.3125645
ACGGCAA855.399797E-511.16111617
GGCGAGG17800.011.11755619
TCGAAAA600.005936229611.06836914
TATACCG600.005981419211.0570965
AAGGCGT1201.3184217E-711.0570966
TGTAGGA40400.010.8616032
CCAACGA1159.205178E-710.703567519
CCTACAC2750.010.683743
GGACCTG22100.010.6782816
AAAGGCG1252.3153734E-710.6148135
GTCCTAC34650.010.5617321
Produced by FastQC (version 0.11.5)