FastQCFastQC Report
Thu 26 May 2016
SRR1512476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121991
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA75430.3554680486392261No Hit
GTCCTACAGTGGACATTTCTAAATT72890.34349815809774875No Hit
GTATCAACGCAGAGTACTTTTTTTT72390.34114188043210364No Hit
GTCCTAAAGTGTGTATTTCTCATTT68290.3218204035738135No Hit
CTTTAGGACGTGAAATATGGCGAGG61450.28958652510778793No Hit
TATCAACGCAGAGTACTTTTTTTTT47450.22361075046972395No Hit
GGTATCAACGCAGAGTACTTTTTTT47260.2227153649567788No Hit
GTCCTACAGTGTGCATTTCTCATTT38910.18336552794050492No Hit
GTACATGGGAAGCAGTGGTATCAAC34510.16263028448282768No Hit
CTGAAGGACCTGGAATATGGCGAGA32200.15174428166754714No Hit
CTGTAGGACCTGGAATATGGCGAGA29680.1398686422326956No Hit
ATTTAGAAATGTCCACTGTAGGACG28080.13232855370263116No Hit
GTCCTTCAGTGTGCATTTCTCATTT26170.12332757301986672No Hit
CCCATGTACTCTGCGTTGATACCAC24770.11672999555606033No Hit
TTTCTAAATTTTCCACCTTTTTCAG23940.11281857463108938No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23430.11041517141213134No Hit
GAATATGGCAAGAAAACTGAAAATC23310.10984966477237651No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21340.10056593076973465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC350.002167009516.2896964
TAGGACC10300.013.8383334
CCAACGA852.7119495E-713.4071419
AAATGTC8900.012.3806717
TCCAACG1800.012.13556118
GGCGAGG16400.011.92881319
AGGACCT22500.011.4875235
TAGAAAT10550.011.4388154
TGTCCAC10200.011.26864310
GAAATGT10100.011.1915846
AATGTCC9600.011.181078
CACCTTT8850.011.16157114
AACACCG600.00586514611.0863045
GGACCTG21800.010.893056
GGACGTG37900.010.8771846
AGGACGT38600.010.8565375
TCGAACT1408.400093E-910.85339919
TGTAGGA39400.010.8285342
ATTTAGA10500.010.7812471
GACGTGG19750.010.5811237
Produced by FastQC (version 0.11.5)