FastQCFastQC Report
Thu 26 May 2016
SRR1512475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71540.29015226301730773No Hit
GTACATGGGAAGCAGTGGTATCAAC66940.27149556173299666No Hit
GTCCTAAAGTGTGTATTTCTCATTT65670.266344689856676No Hit
CTGTAGGACGTGGAATATGGCAAGA64510.2616399564893279No Hit
GTATCAACGCAGAGTACTTTTTTTT54840.22242032574600443No Hit
CTTTAGGACGTGAAATATGGCGAGG53190.21572824811141458No Hit
CCCATGTACTCTGCGTTGATACCAC48410.19634150199423916No Hit
GTCCTACAGTGTGCATTTCTCATTT35740.14495445736984316No Hit
GGTATCAACGCAGAGTACTTTTTTT34290.13907354066065813No Hit
TATCAACGCAGAGTACTTTTTTTTT33270.1349366199410935No Hit
CTGAAGGACCTGGAATATGGCGAGA30750.12471599228099264No Hit
GAGTACATGGGAAGCAGTGGTATCA28680.11632047670305265No Hit
GTCCTTCAGTGTGCATTTCTCATTT26720.1083710996340853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA903.7056452E-813.70414317
TAGGACC11600.013.3985764
AGGACCG957.631024E-812.9685835
AATCCCG1850.012.79638119
GTGTAGG4650.011.7270811
TATTGCG1157.2166586E-811.54927316
AAATGTC10150.011.3911247
TCCAACG1501.8735591E-1011.36156718
TTGCGAG1105.1938696E-711.18942218
GTCCTAA19800.011.1164971
GGACCGG600.005984093511.0565486
GGACCTG23500.011.0498276
CCACCTT10250.011.00956913
GCCGGTT951.3926001E-510.97722111
AGGACCT24700.010.9350485
ACCTTTT10850.010.92983415
AATGTCC10400.010.9094798
GTCCTAC34000.010.8478631
CTAACGT700.001522350610.8309054
GACGTGG22200.010.6296557
Produced by FastQC (version 0.11.5)